西安交通大学学报（医学版）2017，Vol.38Issue(6)：921-925,5.DOI:10.7652/jdyxb201706027
4313份血样细菌培养结果分析
Analysis of 4313 cases of blood culture bacteria
侯轩 1徐纪茹 2辜依海 1陶浚齐 1邓明慧 1王辉1
作者信息
- 1. 西安交通大学医学院附属三二〇一医院微生物免疫科,陕西汉中 723000
- 2. 西安交通大学医学部医学免疫学与病原生物学系,陕西西安 710000
- 折叠
摘要
Abstract
Objective To collect the laboratory parameters and clinical parameters of blood culture positive samples,and analyze the composition and laboratory characteristics of real pathogens by combining with clinical follow-up and anti-infectious treatment outcomes.Methods We conducted a prospective study to isolate the 483 strains of pathogens from 4313 cases of blood samples for clinical examination between March 2013 and March 2015.The results of laboratory and clinical infections were collected for each positive culture and were followed up for clinical follow-up to understand the responsible doctors' experience-based judgment and targeted clinical treatment of antibiotics.After comprehensive analysis we determined the real pathogens and contaminants.Results Of the 483 positive cultures,331 were finally determined as pathogenic ones,accounting for 68.5% of the number of positive isolates; 97 were contaminated bacteria (20.1%); and 55 strains with uncertain pathogenic nature (11.4%).Escherichia coli accounted for the highest proportion (41.2%)of pathogenic bacteria.Coagulase-negative staphylococci took up the highest proportion (75.3%)of the contaminated bacteria.As many as 253 strains (52.4%)were detected from the aerobic or anaerobic bottles.The detection rate of Escherichia coli in anaerobic bottles (23.9%)was higher than that in aerobic bottles (13.8%)(P <0.05).Of 97 strains of positive isolates,only one bottle was reported positive for 90 strains,accounting for (92.8%),and more than two bottles of 7 positive strains,accounting for (7.2%)(P <0.05).34 positive in 24 h (35.1%),77 positive in 48 h (79.4%),the positivebacteria ratio within 48 h (79.4%)was higher than that of bacteria contamination ratio within 24 h (χ2 =38.935, P =0.000),with a significant difference.Conclusion Establishment of contaminated bacteria in blood culture cannot rely solely on laboratory or clinical parameters.It should be combined with the experience of clinicians to determine the clinical response to comprehensive judgments.For the laboratory to determine the presence of contamination,the number of positive bottles and the amount of sun are still two factors of important value.Paying attention to inspection of anaerobic bottles is more conducive to the detection of Escherichia coli.关键词
血培养/污染菌/厌氧瓶/临床参数Key words
blood culture/contaminated bacteria/anaerobic bottle/clinical parameter分类
医药卫生引用本文复制引用
侯轩,徐纪茹,辜依海,陶浚齐,邓明慧,王辉..4313份血样细菌培养结果分析[J].西安交通大学学报（医学版）,2017,38(6):921-925,5.
