西北国防医学杂志2017，Vol.38Issue(9)：574-578,5.DOI:10.16021/j.cnki.1007-8622.2017.09.004
miRNA-210在人脑胶质瘤中表达的临床意义及其生物学效应
Clinical significance and biological effects of miRNA-210 expression in human glioma
摘要
Abstract
Objective:To investigate the expression and clinical significance of microRNA-210 (miRNA-210) in glioma tissues and its effect on the proliferation and invasion ability of glioma cells.Methods:Fourty-two cases of brain glioma and 20 cases of benign meningioma were collected in the department of neurosurgery in our hospital.Quantitative real-time PCR (qPCR) was used to detect the expression of miRNA-210 in tissue of patients,and Kaplan Meier survival was used to analyze the relationship between the expression and the patients prognosis.Small interference RNA (siRNA) was used to interfere the expression of miRNA-210 in U251 human glioma cell line in vitro.MTT assay was used to detect the ability of cell proliferation.Transwell invasion assay was used to detect the ability of cell invasion.Results:The expression of miRNA-210 in 29 cases of high grade gliomas was higher than that of 13 cases of low grade gliomas and 20 cases of benign meningioma (P ＜0.05).Kaplan-Meier survival showed the prognosis of glioma patients with high expression of miRNA-210 was poorer than patients with low expression (P＜0.05).The siRNA was transfected to U25I cells successfully,and the expression of miRNA-210 in U251 siRNA-210 cell-was decreased significantly compared to U251 control.The results of MTT showed the solution relative absorbance of U251 siRNA-210 cells was significantly lower than that of U251 control cell in 72 h (P＜0.05).The results of transwell invasion assay showed the cell number of penetrating membrane in U251 siRNA-210 cells was significantly less than that of U251 control cells.Conclusion:The expression of miRNA-210 is closely related to the malignant degree and prognosis of glioma patients,which can promote the proliferation and invasion of glioma cells.关键词
人脑胶质瘤/miRNA-210/增殖能力/侵袭能力Key words
human glioma/miRNA-210/proliferation ability/invasion ability分类
医药卫生引用本文复制引用
徐鹏翔,李强,许琼冠,张彩彩..miRNA-210在人脑胶质瘤中表达的临床意义及其生物学效应[J].西北国防医学杂志,2017,38(9):574-578,5.基金项目
海南省自然科学基金资助项目(20158308) （20158308）
