陕西科技大学学报2017，Vol.35Issue(5)：145-150,155,7.
短短芽孢杆菌M01产抗菌物质工艺条件优化及其主要抑菌成分探究
Optimization of technological conditions for production antibacterial substances from Brevibacillus brevis M01 and investigation of the main antibacterial components
摘要
Abstract
A new strain of Brevibacillus brevis M01 was carried out to reserach the main com-ponents of the active antibacterial substance and optimize its fermentation conditions .The optimum conditions to produce the antimicrobial substances were as follows :Initial pH6 .0 , inoculation amount 8% ,fermentation temperature 28 ℃ and fermentation cycle 48 h by sin-gle factor and orthogonal experiment ,after optimization ,the activity of antibacterial sub-stances was 38 .7% higher than before .In addition ,the 80% of cold ethanol was used to ex-tract the antimicrobial substance that was desalted and purified by Sephadex-50 gel column , and SDS-PAGE analysis showed that the protein with molecular weight of 62 .0 kD ,40 .0 kD ,29 .0 kD and 20 .1 kD could be the main component of the antimicrobial active substance of strain M01 ,and the protein of about 29 .0 kD may play an important role in it .关键词
短短芽孢杆菌M01/抑菌活性/正交优化/主要抑菌成分Key words
Brevibacillus brevis M01/antimicrobial activity/orthogonal optimization/main antibacterial ingredients分类
生物科学引用本文复制引用
王忠忠,龚国利..短短芽孢杆菌M01产抗菌物质工艺条件优化及其主要抑菌成分探究[J].陕西科技大学学报,2017,35(5):145-150,155,7.基金项目
陕西省教育厅自然科学专项科研计划项目(14JK1101) （14JK1101）
西安市未央区科技计划项目(201309) （201309）
