现代妇产科进展2017，Vol.26Issue(10)：735-738,4.DOI:10.13283/j.cnki.xdfckjz.2017.10.036
缺氧下5-脂氧合酶在卵巢癌中的表达及意义
Expression of 5-LOX in ovarian cancer under hypoxia and its clinical significance
刘慧 1郑桂喜 1邹明瑾 1杜鲁涛 1张欣 1杨咏梅 1张义 1王传新2
作者信息
- 1. 山东大学齐鲁医院检验科,济南 250012
- 2. 山东大学第二医院,济南 250033
- 折叠
摘要
Abstract
Objective:To detect the expression level of 5-LOX in ovarian cancer,ex-plore the correlations between 5-LOX expression and clinicopathological features, and investi-gate its effect on the development and treatment of ovarian cancer. Methods:The expressions of 5-LOX and HIF-1α in 56 ovarian cancer specimens was examined by immunohistochemistry, and the correlations between 5-LOX expression and clinicopathological features were further an-alyzed. The human ovarian cancer SKOV3 cells were cultivated 0h,12h,24h,48h respectively under hypoxia environment, and then RNA and protein were extracted. Quantitative real-time PCR ( qRT-PCR) was used to measure the mRNA expression level of 5-LOX. Western blot was used to measure the protein expression level of 5-LOX. ELISA was used to measure the change of 5-HETE. Result:Immunohistochemistry results showed that 5-LOX level was correlated with HIF-1αlevel,and its level was significantly correlated with lymph nodes metastasis,distant me-tastasis and clinical stage. 5-LOX was up regulation both in transcription and protein levels in o-varian cancer SKOV3 cells under hypoxia. The 5-HETE level was also increased under hypoxia. Conclusions:Hypoxia can promote the expression of 5-LOX and increase its metabolites 5 -HETE in ovarian cancer,suggesting 5-LOX can be a potential target for the treatment of ovarian cancer,which provides a new thought and method in the pathogenesis and treatment for ovarian cancer.关键词
缺氧/卵巢癌/5-脂氧合酶/缺氧诱导因子-1αKey words
Hypoxia/Ovarian cancer/5-LOX/HIF-1α分类
医药卫生引用本文复制引用
刘慧,郑桂喜,邹明瑾,杜鲁涛,张欣,杨咏梅,张义,王传新..缺氧下5-脂氧合酶在卵巢癌中的表达及意义[J].现代妇产科进展,2017,26(10):735-738,4.
