现代妇产科进展2017，Vol.26Issue(10)：744-746,751,4.DOI:10.13283/j.cnki.xdfckjz.2017.10.002
斯钙素1对卵巢上皮细胞恶性转化的影响26
The effect of stanniocalcin-1 on the malignant transformation of ovarian epithelial cells
摘要
Abstract
Objective:To study the effect of STC1 on the malignant transformation of o-varian epithelial cells. Methods:STC1 gene was transfected into immortalized ovarian epithelial cells(T29-STC1)by lentivirus transfection as study group,while control group(T29-Vector) was transfected with empty vector. Western blot was used to detect the expression of STC1 in T29-STC1 cells and T29-Vector cells. Cytometry was used to determine whether the level of STC1 expression affects cell cycle progression. Plate cloning experiments was used to count the number of cell formation after disposed by carboplatin. MTT assay was used to detect the prolif-eration ability of the cells and subcutaneous tumor xenograft in nude mice was used to detect the ability of tumor formation. Results:Western blot showed that the expression of STC1 in T29-STC1 was significantly enhanced. Cytometry showed that the T29-STC1 cells in G0 and G1 cell cycle decreased but increased in S cell cycle in comparision with T29-Vectorcells. Plate cloning experiments and MTT showed that the growth rate of T29-STC1 was significantly higher than that of T29-Vector after carboplatin treatment. T29-STC1 inoculated in nude mice could form tumor while T29-Vector could not. Conclusion:Stanniocalcin-1(STC1)protein may be involved in malignant transformation of ovarian epithelial cells.关键词
斯钙素1/卵巢癌/裸鼠/化疗耐药Key words
Stanniocalcin-1 ( STC1 )/Ovarian cancer/Nude mice/Chemoresistance分类
医药卫生引用本文复制引用
杨舒心,胡方方,刘广芝..斯钙素1对卵巢上皮细胞恶性转化的影响26[J].现代妇产科进展,2017,26(10):744-746,751,4.基金项目
河南省医学科技攻关计划项目(No:201301007) （No:201301007）
