现代妇产科进展2017，Vol.26Issue(10)：752-755,4.DOI:10.13283/j.cnki.xdfckjz.2017.10.004
维生素E干预对GDM孕鼠胎盘长链不饱和脂肪酸转运相关蛋白的影响26
Effects of vitamin E intervention on long chain polyunsaturated fatty acid transport-re-lated proteins in placenta tissue of GDM pregnant rats
摘要
Abstract
Objective:To examine the levels of long chain polyunsaturated fatty acid ( LC-PUFA) transport-related proteins and to explore the role of Vitamin E on them in placenta of pregnant rats with gestational diabetes mellitus ( GDM ) . Method:A total of 36 C57 BL/6 J maternal mice with mating were divided into 3 groups,namely Control group,GDM group and Vitamin E supplement group. We used 24 rats of them to establish a model of gestational diabe-tes mellitus ( GDM) ,then which were averaged into two groups and fed with Vitamin E supple-ment in one group. Vit E group was treated with 5mg/(kg·d) Vitamin E,while GDM and Control group were fed with equal dose of solvent. On the 18th day after pregnancy,the levels of SOD,GSH-px and MAD in the blood were measured,and the levels of PPARγ,FATP-1,FATP-4,FAT/CD36, FABPpm and their mRNA also were detected. Results:The levels of serum SOD,GSH-px and MAD in GDM group were significantly higher than those in control group and Vit E group ( P<0 . 05 ) . The difference between Control group and Vit E group was not signifi-cant ( P>0 . 05 ) . The expressions of PPARγ, FATP-1 and FATP-4 in GDM group were lower than those in control group ( P<0 . 05 ) , this trend was reversed in terms of FAT/CD36 and FABPpm (P<0. 05). Compared with GDM group,the levels of PPARγ,FATP-1 and FATP-4 were significantly higher (P<0. 05),and the expression of FAT/CD36 and FABPpm were sig-nificantly lower (P<0. 05) in VitE group. Conclusion:Vitamin E could reduce oxidative stress in GDM pregnant rats by upregulating the levels of PPARγ,FATP-1 and FATP-4 and down-reg-ulating the levels of FAT/CD36 and FABPpm in placental tissue.关键词
维生素E/妊娠期糖尿病/胎盘/脂肪酸转运蛋白Key words
Vitamin E/Gestational diabetes mellitus/Placental/Fatty acid transport protein分类
医药卫生引用本文复制引用
杨东,胡传来,赵奇红,汪秋伟,孙金华..维生素E干预对GDM孕鼠胎盘长链不饱和脂肪酸转运相关蛋白的影响26[J].现代妇产科进展,2017,26(10):752-755,4.基金项目
国家自然科学基金(No:81373011) （No:81373011）
