西南国防医药2017，Vol.27Issue(10)：1040-1043,4.DOI:10.3969/j.issn.1004-0188.2017.10.002
姜黄素对HepG2细胞增殖及Keap1、Nrf-2表达的影响
Effects of curcumin on HepG2 cell proliferation and expression of Keap1 and Nrf-2
摘要
Abstract
Objective To explore the effects of curcumin on HepG2 cell proliferation and the expression of Keap1 and Nrf-2.Methods HepG2 cells were treated with curcumin (10 μmol/L,20 μmol/L and 40 μmol/L).MTT method was adopted for detecting the cell proliferation activity,real-time PCR method was adopted for detecting the expression of Keap1 mRNA,Western blotting method was adopted for detecting the expression of Keap1 and Nrf-2 protein,and fluorescence microplate was adopted for detecting the changes in the intracellular reactive oxygen species(ROS).Results Curcumin could inhibit the proliferation activity of HepG2cell;there was significant difference between the middle-dose group and low-dose group (P ＜ 0.05),but the difference between the high-dose group and the middle-dose group was not significant(P ＞ 0.05);curcumin could promote the expression of Keap1 mRNA and protein and the expression increased with the increase of drug concentration(P ＜ 0.05);curcumin could inhibit the expression of Nrf-2 protein and the expression decreased with the increase of drug concentration (P ＜ 0.05);the intracellular ROS of HepG2 increased with the increase of curcumin concentration (P ＜ 0.05).Conclusion Curcumin could inhibit the proliferation of HepG2.The mechanism may be correlated to the promotion of Keap1 expression,the inhibition of Nrf-2 nuclear translocation,the weakening of Nrf-2 antioxidant function,the increase of ROS and the acceleration of cell apoptosis.关键词
姜黄素/肝癌/HepG2细胞/细胞增殖/keap1/Nrf-2Key words
curcumin/liver cancer/, HepG2 cell/cell proliferation/keap1/Nrf-2分类
医药卫生引用本文复制引用
王霞,闫军,郭薇..姜黄素对HepG2细胞增殖及Keap1、Nrf-2表达的影响[J].西南国防医药,2017,27(10):1040-1043,4.基金项目
国家自然科学基金面上项目(81372272) （81372272）
