西南国防医药2017，Vol.27Issue(10)：1043-1046,4.DOI:10.3969/j.issn.1004-0188.2017.10.003
HPLC法测定复方益肤乳膏中酮康唑和丙酸氯倍他索含量
Determination of ketoconazole and clobetasol propionate in Compound Yifu Cream by HPLC
摘要
Abstract
Objective To determine the contents of ketoconazole and clobetasol propionate in Compound Yifu Cream by high efficiency liquid chromatography (HPLC) to provide basis for improving and controlling the quality standard for Compound Yifu Cream prepared by our hospital.Methods HPLC was adopted for determining the contents of ketoconazole and clobetasol propionate in Compound Yifu Cream,with methanol-water (75∶25) as mobile phase;the check wavelength was 239 nm for precision,stability and recovery tests.Results Ketoconazole and clobetasol propionate were within the concentration range of 0.323-2.584 mg/ml and 4.107-32.859 μg/ml and were in a good linear relation with respective peak area.Within 24 hours,the precision and stability of detection results were excellent.Sample recovery rate:ketoconazole 102.54％ (RSD=1.2％) and clobetasol propionate 105.16％ (RSD=1.1％).Conclusion HPLC can accurately detect the contents of ketoconazole and clobetasol propionate in Compound Yifu Cream,and the specificity,precision and recovery rate is high;it is suitable for the quality control of the preparation.关键词
高效液相色谱法/复方益肤乳膏/酮康唑/丙酸氯倍他索/含量/测定Key words
HPLC/Compound Yifu Cream/ketoconazole/clobetasol propionate/content/determination分类
医药卫生引用本文复制引用
胡志飞,杜建红,谢晓玲..HPLC法测定复方益肤乳膏中酮康唑和丙酸氯倍他索含量[J].西南国防医药,2017,27(10):1043-1046,4.基金项目
军队医疗机构制剂标准提高科研专项课题(14ZJZ01-3) （14ZJZ01-3）
