信阳师范学院学报（自然科学版）2017，Vol.30Issue(4)：591-595,5.DOI:10.3969/j.issn.1003-0972.2017.04.017
基于GO-AuNPs@Ru(bpy)2+3的无标记型 癌胚抗原电化学发光适配体传感器
Electrochemiluminescence Aptasensor based on GO-AuNPs@Ru(bpy)2+3 Nanocomposite for Detection of Carcinoembryonic Antigen
摘要
Abstract
Graphene oxide (GO)-AuNPs@Ru(bpy)2+3 nanocomposite was prepared by electrostatic interac-tion of AuNPs and Ru(bpy)2+3 .GO-AuNPs @ Ru(bpy)2+3 further reacted with GO to form GO-AuNPs @ Ru (bpy)2+3 which was used as the substrate material for ECL detection.Streptavidin (SA)can strongly bind with 4 biotin molecules to form SA-biotin.The enhanced electrochemiluminescence (ECL)signal was obtained by the combination of SA-biotin with the recognition element such as aptamer.An ECL aptasensor for sensitive detec-tion of carcinoembryonic antigen (CEA)was fabricated based on GO-AuNPs@Ru(bpy)2+3 and SA-biotin.This aptasensor exhibits a linear relation in the range of 1.0 fg/L to 10.0 pg/L with the detection limit of 0.40 fg/L. The proposed method was successfully applied in determination of human serum samples with recoveries of 80. 0%~108.0% and RSDs less than 8.3%.关键词
电化学发光适配体传感器/联吡啶钌/纳米金/氧化石墨烯/癌胚抗原Key words
electrochemiluminescence aptasensor/ruthenium trisbipyridine/gold nanoparticle/graphene ox-ide/carcinoembryonic antigen分类
化学化工引用本文复制引用
王辉,曹俊涛,刘彦明..基于GO-AuNPs@Ru(bpy)2+3的无标记型 癌胚抗原电化学发光适配体传感器[J].信阳师范学院学报（自然科学版）,2017,30(4):591-595,5.基金项目
国家自然科学基金项目(21675136,21375114,21405129) （21675136,21375114,21405129）
河南省科技创新杰出人才项目(2017JR0016) （2017JR0016）
河南省高校青年骨干教师资助计划(2016GGJS-097) （2016GGJS-097）
