医药导报2017，Vol.36Issue(11)：1264-1267,4.DOI:10.3870/j.issn.1004-0781.2017.11.012
姜黄素对糖尿病心肌病大鼠心肌细胞凋亡的影响
Effect of Curcumin on the Apoptosis of Myocardial Cell in Rats of Diabetic Cardiomyopathy
周开珩 1张明2
作者信息
- 1. 浙江省宁波市医疗中心李惠利医院药剂科,宁波 315040
- 2. 浙江省宁波市鄞州二院药剂科,宁波 315100
- 折叠
摘要
Abstract
Objective To investigate the preventive effect of curcumin on rats with myocardial apoptosis caused by type 1 diabetes. After intragastric administration of curcumin,the expression of genes related to apoptosis and the number of apoptotic myocardium cells were detected. Methods Sixty Wistar rats were randomly divided into 4 groups ( 15 rats per group ):diabetes group, normal control group,curcumin low dose and high dose group. Animal model with type 1 diabetes was induced by intraperitoneal single injection of streptozotocin ( STZ) in each group except the normal one. After two weeks of STZ injection,the rats with diabetes were used for this study. The curcumin treatment groups were treated with curcumin via intragastric administration for 16 weeks ( twice a week) . Normal control group and diabetes group were administrated with equivalent volume of saline. After 16 weeks, the experimental animals were sacrificed and their hearts were arrested after 0. 9% sodium chloride solution perfusion. The number of myocardial cell apoptosis was detected by TUNEL staining and the expressions of Bcl-2 and Bax relate to the cell apoptosis were examined by western blot analysis. Results The result of TUNEL staining was confirmed that the apoptosis index in the rats of diabetes group was significantly higher than the normal control group ( P<0.05) . However, there was a significantly decline of apoptosis index in the diabetes rats after the treatment of curcumin ( P<0.05) and the result showed a significant dose dependence of curcumin ( P<0.05) . The results of Western blot analysis confirmed that there was a significant lower expression of Bcl-2 and a higher expression and a higher expression of Bax in the hearts of diabetes rats compared with normal control rats (P<0.01). After the treatment of curcumin, the expression of anti-apoptosis protein Bcl-2 was significantly increased and the expression of pro-apoptosis protein Bax was obviously decreased in the myocardial cells of rats in curcumin treated group compared with the diabetes group (P<0.01). The result was also in a dose-dependent manner (P<0.05). Conclusion The treatment of curcumin can significantly increase the expression of anti-apoptosis protein Bcl-2 and decrease the expression of pro-apoptosis protein Bax,which induced the decrease of myocardial apoptosis in the heart of diabetes rats and could have the effects on the decline of myocardial apoptosis caused by diabetic cardiomyopathy.关键词
姜黄素/心肌病/糖尿病,1型/细胞凋亡Key words
Diabetic cardiomyopathy/Curcumin/Apoptosis分类
医药卫生引用本文复制引用
周开珩,张明..姜黄素对糖尿病心肌病大鼠心肌细胞凋亡的影响[J].医药导报,2017,36(11):1264-1267,4.
