中国康复理论与实践2017，Vol.23Issue(10)：1145-1151,7.DOI:10.3969/j.issn.1006-9771.2017.10.005
电针百会、神庭穴对脑缺血再灌注大鼠学习记忆能力及海马5-羟色胺1A受体表达的影响
Effects of Electroacupuncture at Baihui and Shenting Acupoints on Learning and Memory Ability and Expres-sion of 5-hydroxytryptamine 1A Receptor in Rats with Cerebral Ischemia-Reperfusion
摘要
Abstract
Objective To explore the effect of electroacupuncture at Baihui (DU20) and Shenting (DU24) acupoints on learning and memory ability in rats with middle cerebral artery occlusion and reperfusion (MCAO/R), and the potential mechanisms. Methods A total of 90 healthy male Sprague-Dawley rats were randomly divided into sham group (n=18) and operation group (n=72). The MCAO/R model was established by suture method in the operation group. Finally, 54 qualified rats of the operation group were randomly divided into ischemia group (n=18), electroacupuncture group (n=18) and non-acupoint group (n=18). The electroacupuncture group received electroacupuncture at Baihui (DU20) and Shenting (DU24) for 14 days. The cerebral infarction volume was measured by magnetic resonance imaging (MRI). The learning and memory ability was tested by Morris water maze. The protein expression of 5-hydroxytryptamine 1A (5-HT1A) receptor was detected by immunohistochemistry. Results There was no significant difference in the cerebral infarction volume among three groups before intervention (F=1.678, P>0.05). Compared with the ischemia group and the non-acupoint group, the cerebral infarction volume signif-icantly reduced (P<0.001);the latency significantly shortened (P<0.001) and the times crossing the platform decreased (P<0.05);the expres-sion of 5-HT1A receptor decreased in the left hippocampus (P<0.05) in the electroacupuncture group after intervention. There was no signifi-cant difference in all the indices between the non-acupoint group and the ischemia group after intervention (P>0.05). Conclusion Electroacu-puncture at Baihui (DU20) and Shenting (DU24) could effectively increase the learning and memory ability of MCAO/R rats, which might relate with inhibiting the expression of 5-HT1A receptor in hippocampus.关键词
脑缺血再灌注/电针/学习记忆/5-羟色胺1A受体/大鼠Key words
cerebral ischemia-reperfusion/electroacupuncture/learning and memory/5-hydroxytryptamine 1A receptor/rats分类
医药卫生引用本文复制引用
金昊,陈立典,柳维林,林冰冰,李珑,彭洪卫,张秀峰,宋长明,黄佳,陶静..电针百会、神庭穴对脑缺血再灌注大鼠学习记忆能力及海马5-羟色胺1A受体表达的影响[J].中国康复理论与实践,2017,23(10):1145-1151,7.基金项目
国家自然科学基金项目(No.81403450). （No.81403450）
