中国农业科学2017，Vol.50Issue(16)：3215-3225,11.DOI:10.3864/j.issn.0578-1752.2017.16.016
牦牛DGAT1基因多态性及其与乳质性状关联分析
Polymorphisms of DGAT1 Gene and Their Association with Milk Quality Traits in Yak
摘要
Abstract
[Objective] In this study,polymorphisms of yak DGAT1 (diacylglycerol acyltransferase 1) gene were investigated and their effects on some milk quality traits were estimated so as to enrich the molecular genetic data for yak.[Method] Mutations in intron5-exon7 and intron15-exon17 of DGAT1 gene were checked by PCR-SSCP in 4 yak populations (Garman yak,Tianzhu white yak,Qinghai yak and Wild-blood yak),and the effects of mutations on the milk quality traits were analyzed in Gannan yak.[Result] The c.562+32_c.562+33insCCGCCC was found in intron5-exon7 of yak DGAT1 gene,and allele M and genotype MM were the dominant allele and genotype,and each yak population was moderately or low polymorphic (PIC＜0.5).The 2 SNPs (c.1249-23C ＞ T and c.1336C ＞ T) were found in intron 15-exon 17,and nucleotide substitution c.1336C ＞ T at exon 17 resulted in p.Arg450Cys change.Allele A was the dominant allele,the genotype AA in Gannan yak,Tianzhu white yak and Qinghai yak and AB in Wild-blood yak were the dominant genotype,respectively.Each yak population was moderately polymorphic (0.25＜PIC＜0.5).Total 6 haplotypes and 11 haplotype combinations were constituted by mutations in these two regions,and there was a linkage relationship between 3 mutations and closed to linkage equilibrium.The association analysis showed that the genotype in intron5-exon7 was not significantly correlated with the milk quality traits in Gannan yak.The milk quality traits of genotype BB were the lowest,and the milk protein rate,fat rate,total solids percentage were lower than those of other genetypes (P＜0.05),and individuals with possessing allele A had higher milk protein rate,fat rate,total solids percentage and non-fat solids percentage than those no-possessing (P＜0.05),and yaks with allele C also showed higher milk fat rate than those no-possessing (P＜0.05),but individuals with allele B showed lower milk fat rate and total solids percentage than those no-possessing (P＜0.05) in intron15-exon17 ofDGAT1 gene in Gannan yak.Individuals with haplotype combination H1H3 had higher milk protein rate,fat rate,total solids percentage and non-fat solids percentage,and the milk fat rate and total solids percentage were higher than those of other haplotype combinations (P＜0.05),while those with haplotype combination H2H2 were lower,in addition to the lactose rate,the other traits were lower than other combinations (P＜ 0.05).[Conclusion] The intron5-exon7 and intron 15-exonl 7 of yak DGAT1 gene are low and moderately polymorphic,and the c.562+32_c.562+33insCCGCCC in intron6 and 2 SNPs (c.1249-23C＞T,c.1336C ＞ T) in intron 15 and exon17 were detected,respectively.The mutations in intron 15-exon1 7 of DGAT1 gene may affect some milk quality waits of Gannan yak,and selecting yak with allele A and haplotype H1H3,or eliminating ones with allele B and haplotype H2H2 can improve the milk quality of Gannan yak.关键词
牦牛/DGAT1基因/多态性/乳品质性状Key words
yak/DGAT1 gene/polymorphism/milk quality traits引用本文复制引用
高小莉,胡江,郭淑珍,石斌刚,谢建鹏,罗玉柱,王继卿,牟永娟..牦牛DGAT1基因多态性及其与乳质性状关联分析[J].中国农业科学,2017,50(16):3215-3225,11.基金项目
国家自然科学基金(31160451)、甘肃省农业生物技术研究与应用开发项目(GNSW-2013-9及GNSW-2016-29)、甘肃省杰出青年科学基金(1210RJDA008) （31160451）
