中国实验动物学报2017，Vol.25Issue(5)：467-472,6.DOI:10.3969/j.issn.1005-4847.2017.05.001
miR-5572转基因小鼠构建病态窦房结综合征疾病模型的繁殖与鉴定
Breeding and genotyping of a miR-5572transgenic mouse model of sick sinus syndrome
摘要
Abstract
Objective This study aimed to explore the possibility of establishing a model of sick sinus syndrome by using miR-5572 transgenic mice. Methods F1 and F2 miR-5572 transgenic mice were bred and genotyped, and then observed the phenotype levels of miR-5572 transgenic mice by morphology, electrocardiogram record ( ECG ) and the Cav1. 2 and Cav1. 3 expressions levels of mRNA and protein in sinoatrial node tissue of homozygous, heterozygote and wild type mice. Results Compared with the wild type and heterozygous mice,the miR-5572 homozygous mice showed a devel-opment delay and smaller body shape,and had slower average heart rate. The mRNA and protein levels of Cav1. 2 and Cav1. 3 in the sinoatrial node tissues were significantly lower. Conclusions The results of this study indicate that miR-5572 homozygous mice may be an efficient approach to establish the model of sick sinus syndrome关键词
病态窦房结综合征/转基因小鼠/繁殖与鉴定/微小RNA-5572Key words
Sick sinus syndrome/Transgenic mice/Breeding and genotyping/miR-5572分类
生物科学引用本文复制引用
张进,李颖,范洁..miR-5572转基因小鼠构建病态窦房结综合征疾病模型的繁殖与鉴定[J].中国实验动物学报,2017,25(5):467-472,6.基金项目
国家自然科学基金项目(81360039,81260038) （81360039,81260038）
云南省心律失常诊治研究中心基金(No. 2014NS260, 2014NS259). （No. 2014NS260, 2014NS259）
