中国实验动物学报2017，Vol.25Issue(5)：479-485,7.DOI:10.3969/j.issn.1005-4847.2017.05.003
急性高血糖影响小鼠第一时相胰岛素分泌的机制
Impairment mechanisms of acute hyperglycemia in the first-phase insulin secretion in mouse
摘要
Abstract
Objective To clarify the impairment mechanisms of acute hyperglycemia in the first-phase insulin se-cretion in mice. Methods The mouse model of acute glucose toxicity was established by glucose infusion through jugular vein catheterization. The glucose and insulin levels were assessed by IPGTT and OGTT in the mice of acute hyperglycemia and control groups. The histology of pancreatic islets was observed using HE staining and the insulin granules and other cy-toplasmic organelles were observed by electron microscopy. Results The mouse model of acute hyperglycemia was suc-cessfully established. The IPGTT showed that the blood glucose level was decreased by 87% ( 10. 3 ± 0. 33 mmol/L vs. 19. 3 ± 1. 66 mmol/L) at 15 min in the acute hyperglycemia group compared with the control group. The OGTT showed that the blood glucose level was decreased by 85% (9. 8 ± 0. 31 mmol/L vs. 18. 16 ± 1. 01 mmol/L) at 30 min in the acute hy-perglycemia group compared with the control group. However, the peak values of insulin secretion were delayed in both IPGTT and OGTT. Insulin levels at 2. 8 and 16. 7 mmol/L glucose stimulation in the acute hyperglycemia group was de-clined by 46% and 67% than the control group, respectively (P<0. 05). Residual insulin content in isletβcells was de-clined by 49% at 2. 8 mmol/L and 94% at 16. 7 mmol/L glucose infusion than the control group (P<0. 05). The histolo-gy showed irregular structure of pancreatic islets in the acute hyperglycemia group. The electron microscopy revealed that the amount of insulin granules was decreased, and more cytoplasmic vacuoles and swollen mitochondria were observed. Conclusions Acute intravenous glucose load decreases insulin content of isletβcells, leading to decrease and delay of the first-phase insulin secretion.关键词
胰岛/急性高血糖试验/糖耐量试验/胰岛素分泌/组织学/超微结构/小鼠Key words
Acute hyperglycemia/Pancreatic islets/Glucose tolerance test/Insulin secretion/Histology/Ultra-structure/Mouse分类
生物科学引用本文复制引用
赵一楠,周迎生,颜迪恩,高秀莹..急性高血糖影响小鼠第一时相胰岛素分泌的机制[J].中国实验动物学报,2017,25(5):479-485,7.基金项目
国家自然科学基金资助项目( 81641027 ) （ 81641027 ）
教育部留学回国基金资助项目( 2012-940 ) （ 2012-940 ）
北京市科委科研基金资助项目(Z131100004013044) （Z131100004013044）
北京市学科带头人基金资助项目(2013-2-006). （2013-2-006）
