中国实验动物学报2017，Vol.25Issue(5)：486-493,8.DOI:10.3969/j.issn.1005-4847.2017.05.004
新生小鼠缺氧缺血性脑损伤模型的建立
Establishment of a neonatal mouse model of hypoxic-ischemic brain damage
摘要
Abstract
Objective To improve the classic Vannucci method for establishing a model of hypoxic-ischemic brain damage in the neonatal mice. Methods Postnatal day 11 KM mice were randomly assigned into normal control group ( N group, n=20) and hypoxic-ischemic brain damage group (HIBD group, n=160). For the HIBD group, the left common carotid artery of mice was ligated and exposed to hypoxia according to different conditions in the groups C1-C8, then com-pared the mortality and the success rates of all groups. TTC staining and relative infarct volume was measured to select the most stable conditions of modeling. In all groups, the growth and development of mice were evaluated by body weight growth curve at different time points after modeling. Longa test, grip test and hanging test were porformed to assess the neu-romotor function. HE staining was used to detect cerebral neuronal pathological changes. Results Neonatal mouse models of hypoxic-ischemic brain damage were established by the left common carotid artery ligation and hypoxia for 45 min under conditions of 8% O2 and 35℃, which resulted a low mortality rate (8. 3%) and high success rate (47. 92%). Compared with the normal group, mice of the HIBD group grew slowly in body weight and showed severe motor dysfunction. The liga-tion side of cerebral artery showed infarction area which accounted for 7. 76 ± 0. 70% of the total brain. The cortex and hip-pocampus of ligated brain tissue showed neuron degeneration and necrosis. Conclusions The neonatal mouse model of hy-poxic-ischemic brain damage is successfully established by our modified method , i. e. to ligate the left common carotid ar-tery and to expose the mice to hypoxia at 8% O2 and 35℃ for 45 min. This model provides a liable and stable experimental animal model for research of neonatal hypoxic-ischemic brain damage.关键词
缺氧缺血/脑损伤/动物模型/新生小鼠Key words
Hypoxia-ischemia/Brain damage/Animal model/Neonatal mice分类
生物科学引用本文复制引用
张丹,周玉陶,徐玉庭,李志荣,虎鑫,王茜,李虹椿,李凡..新生小鼠缺氧缺血性脑损伤模型的建立[J].中国实验动物学报,2017,25(5):486-493,8.基金项目
国家自然科学基金项目(No. 81200939 （）
No. 31260242) （）
云南省科技计划项目(No. 2011FB060) （No. 2011FB060）
2016年度昆明医科大学大学生创新性实验计划项目(No. 201602). （No. 201602）
