中国实验动物学报2017，Vol.25Issue(5)：506-512,7.DOI:10.3969/j.issn.1005-4847.2017.05.007
骨内单次注射小剂量辛伐他汀对大鼠心梗后血管新生的影响
Effect of single intraosseous injection of simvastatin on neoangiogenesis in myocardial infarction in rats
摘要
Abstract
Objective To explore the effect of single local intraosseous injection of small dose simvastatin on the angiogenesis and cardiac function in rats after myocardial infarction. Methods Adult male Wistar rats were divided into sham operation group, myocardial infarction model group and intraosseous injection of simvastatin 0. 5 mg group ( all n=12 per group) . The left anterior descending branch of coronary artery was ligated to establish a rat model of myocardial infarc-tion. The left ventricular function was evaluated by small animal echocardiography at 4 weeks postoperatively. The rest of the rats were sacrificed, the myocardial infarct size was evaluated by TTC staining, and the myocardial neovascularization was detected by immunofluorescence staining. Results We successfully established the rat model of myocardial infarction. The echocardiography showed that the left ventricular systolic function was decreased significantly at 4 weeks after myocardi-al infarction. Intraosseous injection of simvastatin (0. 5 mg) did not improve the left ventricular function after myocardial infarction in the rats. TTC staining showed that intraosseous injection of simvastatin did not reduce myocardial infarct size. Immunofluorescence staining showed that the myocardial capillary density of simvastatin group was slightly higher than that of myocardial infarction model group, but showing no significant difference between them. Conclusions Intraosseous in-jection of simvastatin 0. 5 mg 24 hours after myocardial infarction cannot significantly promote myocardial angiogenesis, which is believed to be beneficial to the revascularization after ischemia, and thus failed to improve the cardiac function.关键词
骨内注射/辛伐他汀/血管新生/心肌梗死/内皮祖细胞Key words
Intraosseous injection/Simvastatin/Neovascularization/Myocardial infarction/Endothelial progenitor cells分类
生物科学引用本文复制引用
刘灿,海宝,张稳,祝俊雄,王红,李子健,徐迎胜,宋纯理..骨内单次注射小剂量辛伐他汀对大鼠心梗后血管新生的影响[J].中国实验动物学报,2017,25(5):506-512,7.基金项目
国家自然科学基金(NO. 81672133,81641079) （NO. 81672133,81641079）
国家高技术研究发展计划(863计划NO. 2015AA020304). （863计划NO. 2015AA020304）
