中国实验动物学报2017，Vol.25Issue(5)：513-518,6.DOI:10.3969/j.issn.1005-4847.2017.05.008
小鼠小肠类器官体外培养体系的建立与应用
Establishment and application of an in vitro mouse intestinal organoid culture system
摘要
Abstract
Objective To establish a small intestinal organoid culture system as an in vitro study model of intesti-nal epithelial cells, and to explore the relevant pathological detection techniques and provide a convenient platform for in vitro study of various intestinal diseases. Methods The mouse intestinal epithelium was isolated and cultured into or-ganoids to simulate the growth and development of intestinal epithelium in vitro. The proliferation and differentiation signals were detected by immunohistochemistry and three-dimensional immunofluorescence technique. Results The culture system of the mouse small intestine epithelium was established. Immunohistochemical staining and three-dimensional immunofluo-rescence technique were successfully used to detect the growth and development of small intestinal organoids. Conclusions The successfully established mouse small intestinal organoid culture system and application of immunoassay technology will gradually become a most favorable technical means for studies of various intestinal diseases.关键词
小肠类器官/石蜡切片/免疫组化/免疫荧光/小鼠Key words
Small intestinal organoids/Pathology/Immunohistochemistry/Immunofluorescence/Mouse分类
生物科学引用本文复制引用
万光升,张新艳..小鼠小肠类器官体外培养体系的建立与应用[J].中国实验动物学报,2017,25(5):513-518,6.基金项目
国家自然科学基金项目 (No. 313714552014-2017). （No. 313714552014-2017）
