中国比较医学杂志2017，Vol.27Issue(10)：1-6,6.DOI:10.3969.j.issn.1671-7856.2017.010.001
骨内单次注射辛伐他汀促进小鼠乳腺癌肿瘤血管正常化的研究
Study on the effect of single dose intraosseous injection of simvastatin on tumor vascular normalization in murine breast cancer
摘要
Abstract
Objective To investigate the effect of single dose intraosseous injection of simvastatin on tumor vascular structure and function in murine breast cancer. Methods BALB/c mice and 4T1 murine breast cancer cells were used to establish a subcutaneous xenograft model. The mouse model of orthotopic breast cancer received intraosseous injection of a single dose of simvastatin (50 μg) or vehicle only. Frozen tumor tissue sections were prepared for co-immunostained with CD31 andα-SMA. Evans blue dye was injected into the tail vein to observe the vascular permeability. The expression level of HIF-1αwas detected by immunohistochemistry. Results Immunofluorescence dual staining showed that intraosseous injection of simvastatin increased the number of perivascular pericytes in the tumor vessel(P < 0. 05), Evans blue dye content showed that in vivo vessel permeability in the tumor tissue was significantly decreased(P < 0. 05), and the immunohistochemistry results showed that local hypoxic area was significantly improved. Conclusions Single dose intraosseous injection of simvastatin can promote the normalization of tumor vasculature by improving the coverage of pericytes.关键词
辛伐他汀/骨内注射/血管正常化/乳腺癌/周细胞Key words
Simvastatin/Intraosseous injection/Vasculature normalization/Breast cancer/Pericyte分类
医药卫生引用本文复制引用
海宝,刘灿,张稳,祝俊雄,曹宝山,宋纯理..骨内单次注射辛伐他汀促进小鼠乳腺癌肿瘤血管正常化的研究[J].中国比较医学杂志,2017,27(10):1-6,6.基金项目
国家自然科学基金项目(81672133,81641079) （81672133,81641079）
国家高技术研究发展计划(2015AA020304). （2015AA020304）
