中国比较医学杂志2017，Vol.27Issue(10)：10-15,22,7.DOI:10.3969.j.issn.1671-7856.2017.10.003
流式细胞术分析PMA诱导THP-1分化为巨噬细胞的表型特征
Phenotypic characterization of PMA-induced THP-1 macrophages analyzed by flow cytometry
摘要
Abstract
Objective To identify the characteristics of the subtype of PMA-induced THP-1 macrophages by flow cytometry analysis. Methods THP-1 monocytic cells differentiate into macrophages promoted by PMA, then induced into M1 and M2 by adding different cytokines, such as LPS,IL-6 and IFN-γ for THP-1-M1, IL-4,IL-13 and IL-6 for THP-1-M2. Morphology of cells were observed under a microscope and the expression of CD14, CD68, CD16, CD80, CD86, CD163, CD206, CD209, CD83, CD1a, CD11c, HLA-DR were detected by flow cytometry. Results The macrophages stimulated by PMA became adherent;THP-1-M1 and THP-1-M2 lost their spherical morphology, appeared more irregular with many obvious projections. The expression of CD83, CD1a, CD11c, HLA-DR which had the function of antigen presenting on the surface of THP-1-Mφwere very low, and most of them were negative, but those of THP-1-M1 and THP-1-M2 were very high. Conclusions The macrophages differentiated from THP-1 stimulated by PMA are weak in antigen presenting function.关键词
THP-1/巨噬细胞/佛波酯/流式细胞术Key words
THP-1/Macrophage/Phorbol 12-myristate 13-acetate/PMA/Flow cytometry分类
医药卫生引用本文复制引用
彭卓颖,李想,丛喆,薛婧..流式细胞术分析PMA诱导THP-1分化为巨噬细胞的表型特征[J].中国比较医学杂志,2017,27(10):10-15,22,7.基金项目
国家自然科学基金-青年科学基金项目(81301437) （81301437）
科技部重大专项(2014ZX10001001-001-004,2014ZX10001001-002-006). （2014ZX10001001-001-004,2014ZX10001001-002-006）
