中国现代医学杂志2017，Vol.27Issue(24)：11-16,6.DOI:10.3969/j.issn.1005-8982.2017.24.003
二氢杨梅素对裸鼠腹腔移植人胃癌细胞中 Caveolin-1表达的影响
Effect of Dihydromyricetin on caveolin-1 in nude mouse models of human gastric cancer
摘要
Abstract
Objective To investigate inhibitory effect of Dihydromyricetin (DMY) on the growth of gastric cancer and expression of caveolin-1 in nude mouse models of human gastric cancer. Methods Nude mouse models of peritoneal transplanted human gastric cancer was established. Twenty-four successfully transplanted nude mice were randomly divided into 4 groups: tumor group, tumor with low dose group [50 mg/(kg·d)], moderate dose group [100 mg/(kg·d)] and high dose group [200 mg/(kg·d)] of DMY. The growth rate and tumor size were monitored. The expression levels of caveolin-1 and Ki67 in tumors were tested by Immunohistochemistry and Western blot. Results The number, weight, and volume of tumors were significantly decreased in both tumor with moderate and high dose groups dose dependently when compared with the tumor group ( P<0.05). The appetite, activity and mental state of animals in the tumor with moderate and high dose groups were significantly improved in comparison with the tumor group. Immunohistochemistry and Western blot data indicated that the percentage of Caveolin-1 positive cells and expression level of protein Caveolin-1 were significantly increased in the tumor with moderate and high dose groups when compared with the tumor group dose dependently ( P<0.05). Similarly, the percentage of Ki67 positive cells and expression level were significantly increased in the moderate and high dose groups when compared with the tumor group dose dependently ( P<0.05). Conclusions DMY inhibits the growth of peritoneal transplanted human gastric cancer in dose dependent manner and the mechanism may be related to upregulation of Caveolin-1.关键词
二氢杨梅素/胃癌/裸鼠/腹腔移植瘤/小凹蛋白-1Key words
Dihydromyricetin/gastric cancer/nude mice/peritoneal transplantable tumor/Caveolin-1分类
医药卫生引用本文复制引用
王佑权,张帆,周寿红..二氢杨梅素对裸鼠腹腔移植人胃癌细胞中 Caveolin-1表达的影响[J].中国现代医学杂志,2017,27(24):11-16,6.基金项目
湖南省教育厅科研项目(No:11c1108) （No:11c1108）
