中国现代医学杂志2017，Vol.27Issue(24)：17-21,5.DOI:10.3969/j.issn.1005-8982.2017.24.004
水飞蓟素对大鼠肾脏缺血再灌注损伤的
保护作用及机制探讨
Protective effect of Silymarin on renal ischemia-reperfusion injury in rats
董建 1杨洁 1史国辉1
作者信息
- 1. 华北理工大学附属医院 肾内科,河北 唐山 063000
- 折叠
摘要
Abstract
Objective To investigate the protective effect of Silymarin on renal ischemia-reperfusion injury (IRI) and its underlying inhibiting mechanism towards nuclear factor κB (NF-κB). Methods A total of 30 male sprague-dawley (SD) rats were randomly divided into three groups: sham operation (S) group, IRI (IR) group and IRI plus Silymarin (SM) group. Serum creatinine (Cr) and blood urea nitrogen (BUN) were analyzed with automatic biochemical analyzer. Hematoxylin-eosin (HE) staining was performed for histological grading of kidney injury. Expression of interleukin 6 (IL-6) and NF-κB p65 was identified by Immunohistochemical staining and Enzyme-linked immunosorbent assay (ELISA). Results Circulating Cr, BUN, NF-κB and IL-6 were elevated significantly in the IR group compared with those in the S group ( P<0.05), and those in the SM group ( P< 0.05). Animals in the IR group experienced obvious increase of renal tubular damage and expression of IL-6 and NF-κB, which were ameliorated with treatment of Silymarin. Conclusions Inactivation of NF-κB signaling pathway mediates protective effect of Silymarin on renal ischemia-reperfusion.关键词
水飞蓟素/大鼠/缺血再灌注损伤/NF-κBKey words
Silymarin/rat/ischemia-reperfusion injury/NF-κB分类
医药卫生引用本文复制引用
董建,杨洁,史国辉..水飞蓟素对大鼠肾脏缺血再灌注损伤的
保护作用及机制探讨[J].中国现代医学杂志,2017,27(24):17-21,5.
