中国现代医学杂志2017，Vol.27Issue(24)：22-28,7.DOI:10.3969/j.issn.1005-8982.2017.24.005
p38δ在宫颈癌细胞中的表达及其对
缺氧HeLa细胞凋亡的影响
Role of p38δ on hypoxia-induced apoptosis in cervical cancer cells and its effect on hypoxia-mediated apoptosis of HeLa
沈祥丽 1柳怡 1张勇武 1张勇2
作者信息
- 1. 四川省成都市锦江区妇幼保健院 妇产科,四川 成都 610016
- 2. 四川省绵阳市中心医院 妇产科,四川 绵阳 621000
- 折叠
摘要
Abstract
Objective To investigate the expression level of p38δ in cervical cancer cells and its effect on hypoxia-mediated apoptosis of HeLa cells. Methods The expression level of p38δ was identified by Western blot. HeLa cell models stably expressing p38δ were established with lentivirus system. Hypoxia-induced apoptosis in HeLa cells was measured by Annexin V-FITC/PI double labeling system. Results p38δ was significantly down-regulated in cervical cancer tissures and HeLa cells compared with that in normal cervical tissues and non-tumor cell lines, respectively ( P<0.05). Expression of p38δ in cervical cancer tissues was significanthy lower than that in normal cervical tissues. HeLa cells stably expressing p38δ experienced a higher hypoxia-induced apoptosis when compared with control HeLa cells ( P<0.05). Under hypoxia conditions, the expression levels of p53 and Bax in HeLa cells stably expressing p38δ were significantly increased, and Bcl-2 was decreased when compared with those in control HeLa cells ( P< 0.05). Conclusions p38δ is downregulated in cervical cancer. Overexpression of p38δ promotes hypoxia-induced apoptosis.关键词
宫颈癌/HeLa细胞/p38δ/细胞凋亡/缺氧Key words
cervical cancer/HeLa cells/p38δ/apoptosis/hypoxia分类
医药卫生引用本文复制引用
沈祥丽,柳怡,张勇武,张勇..p38δ在宫颈癌细胞中的表达及其对
缺氧HeLa细胞凋亡的影响[J].中国现代医学杂志,2017,27(24):22-28,7.
