中国现代医学杂志2017，Vol.27Issue(24)：29-33,5.DOI:10.3969/j.issn.1005-8982.2017.24.006
miR-148a对食管癌细胞迁移及侵袭的影响
及潜在分子机制研究
Role and potential mechanism of miR-148a in migration and invasion of esophageal carcinoma
李张维 1李涛1
作者信息
- 1. 西南医科大学 临床医学院,四川 泸州 646000
- 折叠
摘要
Abstract
Objective To investigate the expression of miR-148a in esophageal squamous cell carcinoma (ESCC) and its role in cell migration and invasion. Methods A total of 48 cases of ESCC and tumor adjacent tissue ribonucleic acid (RNA) samples were collected in our hospital. Complementary deoxyribonucleic acid (cDNA) was synthetized by reverse transcription polymerase chain reaction (RT-PCR). The expression level of miR-148a was detected by real-time PCR. MiR-148a mimics were synthesized and transfected into TE-1 cells. Migration and invasion rates were determined by wound-healing assay and Transwell assay, respectively. The expression levels of c-myc and matrix metalloprotein 9 (MMP-9) were measured by Western blot. Results miR-148a was down-regulated in ESCC tissue ( P=0.031). Over-expression of miR-148a inhibited migration and invasion rates of TE-1 cells ( P=0.021 and 0.018, respectively). Moreover, protein levels of c-myc and MMP-9 were decreased with miR-148a over expression ( P=0.037 and 0.026, respectively). Conclusions miR-148a inhibits cell migration and invasion of ESCC through inhibition of c-myc/MMP-9 signal pathway.关键词
MiR-148a/食管癌/迁移/侵袭/原癌基因(c-myc)/基质金属蛋白酶9Key words
miR-148a/esophageal cancer/migration/invasion/c-myc/matrix metalloproteinases 9分类
医药卫生引用本文复制引用
李张维,李涛..miR-148a对食管癌细胞迁移及侵袭的影响
及潜在分子机制研究[J].中国现代医学杂志,2017,27(24):29-33,5.
