中国现代医学杂志2017，Vol.27Issue(24)：34-38,5.DOI:10.3969/j.issn.1005-8982.2017.24.007
非小细胞肺癌恶性胸水中癌细胞EML4-ALK重排检测及其临床意义
Detection of gene rearrangement in NSCLC cells and its clinical significance
王莉 1韩永红 1曾娟 1李军1
作者信息
- 1. 华中科技大学同济医学院附属武汉中心医院 肿瘤科,湖北 武汉 430014
- 折叠
摘要
Abstract
Objective To investigate the application of detecting echinoderm microtubule-associated protein-like 4-anaplastic lymphoma kinase (EML4-ALK) gene rearrangement by qRT-PCR in non-small cell lung cancer cells (NSCLC), and to evaluate the relationship between EML4-ALK gene rearrangement and clinical relevance. Methods A total of 138 patients confirmed with NSCLC in malignant pleural effusion (MPE) were recruited in this study. Cancer cells in MPE were identified by Wright's staining and microfluidic chip technology.EML4-ALK gene rearrangement in cancer cells was determined by Immunohistochemistry (IHC), and quantitative real time polymerase chain reaction (qRT-PCR). Results Ten out of 138 (7.25%) patients were identified with EML4-ALK rearrangement by IHC, who were also verified by qRT-PCR. Sensitivity and specificity of IHC and qRT-PCR were 100%. The EML4-ALK gene rearrangement was correlated with EGFR mutation ( P<0.05), while no relation between the EML4-ALK gene rearrangement and tumor characters including histological type, site of primary onset, clinical stage and lymphatic metastasis ( P>0.05). Conclusions qRT-PCR is a reliable method for detection of EML4-ALK rearrangement detection in NSCLC.关键词
非小细胞肺癌/恶性胸水/棘皮动物微管相关类蛋白4和间变性淋巴瘤激酶Key words
non-small-cell lung cancer/malignant pleural effusions/echinoderm microtubule-associated protein-like4 -anaplastic lymphoma kinase分类
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王莉,韩永红,曾娟,李军..非小细胞肺癌恶性胸水中癌细胞EML4-ALK重排检测及其临床意义[J].中国现代医学杂志,2017,27(24):34-38,5.
