中国中医药信息杂志2017，Vol.24Issue(11)：58-62,5.DOI:10.3969/j.issn.1005-5304.2017.11.014
HPLC指纹图谱结合主成分分析对不同产地头顶一颗珠质量的评价
Evaluation of Quality Coherence of Trillium tschonoskii Maxim. from Different Producing Areas Based on HPLC Fingerprint and PCA
摘要
Abstract
Objective To evaluate the quality coherence of Trillium tschonoskii Maxim.from different producing areas by HPLC fingerprint and PCA; To provide a method for quality control. Methods Samples were separated by Hibar C18 (4.6 mm × 250 mm, 5 μm) with acetonitrile-water as gradient mobile phase at the flow rate of 1.0 mL/min. The wavelength was 203 nm and the temperature was 30 ℃. Chromatographic Fingerprint Similarity Evaluation System and PCA were used to analyze the data. Results The results of method validation of HPLC fingerprint met technical standards.15 common peaks was verified and the similarities of 14 batches of Trillium tschonoskii Maxim. from different producing areas were among 0.389–0.979. 3 principal components with the characteristic root cumulative contribution rate reaching 87.674% were screened out by PCA results. The composite score of S2 was the highest (4.926), and the quality was the best. Conclusion The application of HPLC combined with PCA can objectively and effectively evaluate the quality difference of Trillium tschonoskii Maxim.from different producing areas.关键词
头顶一颗珠/指纹图谱/高效液相色谱法/主成分分析/相似度分析Key words
Trillium tschonoskii Maxim./fingerprint/HPLC/principal component analysis/similarity analysis分类
医药卫生引用本文复制引用
陈洁,陈文茜,郭文鼎,雷滔,欧阳俊摇,赵晖,邹海艳..HPLC指纹图谱结合主成分分析对不同产地头顶一颗珠质量的评价[J].中国中医药信息杂志,2017,24(11):58-62,5.基金项目
北京市教育委员会科技发展计划面上项目(KM201510025011) （KM201510025011）
北京市属高等学校青年拔尖人才培育计划(CIT&TCD201404175) （CIT&TCD201404175）
