中国中医药信息杂志2017，Vol.24Issue(11)：63-66,4.DOI:10.3969/j.issn.1005-5304.2017.11.015
高乌头药材常规项检查及高乌甲素、冉乌头碱含量测定
Routine Examination of Aconitum sinomontanum Nakai and Content Determination of Lappacontine and Ranaconitine
摘要
Abstract
Objective To study the routine examination of Aconitum sinomontanum Nakai and determine the contents of lappacontine and ranaconitine; To provide basis for establishing the quality standard of Aconitum sinomontanum Nakai.Methods Aconitum sinomontanum Nakai were collected from different areas.A method of TLC was used for qualitative discrimination. The methods in the Chinese Pharmacopoeia were adopted for the determination of moisture content, ash content and extractives. Determination of lappacontine and ranaconitine were performed by HPLC. Results The TLC showed that the spots were clear and the separation was good. Individual provisional standards:the moisture,total ash and acid-insoluble ash content of Aconitum sinomontanum Nakai were not more than 11.0%, 12.0%, and 7.0%, respectively; water soluble and alcohol soluble extractives were not less than 18.2% and 10.6%,respectively.The content of ranaconitine and lappacontine in Aconitum sinomontanum Nakai were not less than 0.125% and 0.815%, respectively. Conclusion The method established by the study is accurate and reliable,and can be used for quality evaluation of Aconitum sinomontanum Nakai.关键词
高乌头/常规检查/高效液相色谱法/高乌甲素/冉乌头碱Key words
Aconitum sinomontanum Nakai/routine examination/HPLC/lappacontine/ranaconitine分类
医药卫生引用本文复制引用
张立军,戴海蓉,樊秦,夏鹏飞,沈秉学,李芸..高乌头药材常规项检查及高乌甲素、冉乌头碱含量测定[J].中国中医药信息杂志,2017,24(11):63-66,4.基金项目
国家自然科学基金(81560650) （81560650）
甘肃省自然科学基金(1107RJZA242) （1107RJZA242）
