中国中医药信息杂志2017，Vol.24Issue(11)：75-77,3.DOI:10.3969/j.issn.1005-5304.2017.11.018
康咳灵合剂中重楼皂苷Ⅰ、Ⅱ含量测定
Content Determination of Polyphyllin Ⅰ and Ⅱ in Kangkeling Mixture by HPLC
摘要
Abstract
Objective To establish a method for determining the contents of polyphyllin Ⅰ and polyphyllin Ⅱ in Kangkeling Mixture. Methods The contents of polyphyllin Ⅰ and Ⅱ were determined by HPLC gradient elution. Poroshell 120 Ec-C18 column (4.6 mm × 150 mm, 4 μm) was used; Acetonitrile-water (A:B) was set as the mobile phase; the flow rate was 1.0 mL/min; the detection wavelength was 210 nm; column temperature was 30 ℃. Results Polyphyllin Ⅰ showed good linear relationship in the range of 1.009–10.09 μg (r=0.999 6), and the average recovery was 97.31% (RSD=2.05%, n=6). Polyphyllin Ⅱ showed good linear relationship in the range of 0.640 5–6.405 μg (r=0.999 8), and the average recovery was 96.41% (RSD=1.67%, n=6). Conclusion The method is simple, with good repeatability and accurate results, which can be used to determine the contents of polyphyllin Ⅰ and Ⅱ in Kangkeling Mixture.关键词
康咳灵合剂/高效液相色谱法/重楼皂苷Ⅰ/重楼皂苷ⅡKey words
Kangkeling Mixture/HPLC/polyphyllinⅠ/polyphyllinⅡ分类
医药卫生引用本文复制引用
彭敏,石美琴,童晓东,杨水英,许彦..康咳灵合剂中重楼皂苷Ⅰ、Ⅱ含量测定[J].中国中医药信息杂志,2017,24(11):75-77,3.基金项目
南通市卫生和计划生育委员会科技项目(WQ2015065) （WQ2015065）
