中国中医药信息杂志2017，Vol.24Issue(11)：78-81,4.DOI:10.3969/j.issn.1005-5304.2017.11.019
样品处理方法对皖南葛根和粉葛中总黄酮和葛根素含量的影响
Effects of Different Sample Preparation Methods on Total Flavonoids and Puerarin Content from Puerariae Lobatae Radix and Puerariae Thomsonii Radix in South Anhui Province
摘要
Abstract
Objective To establish UV spectrophotometry and HPLC methods for content determinations of total flavonoids and puerarin from Puerariae Lobatae Radix and Puerariae Thomsonii Radix; To compare the ultrasonic method at room temperature, conventional refluxing method and ultrasonic method at heating conditions at the aspect of content determinations. Methods The content determinations of total flavonoids was determined by UV spectrophotometry at 250 nm; the content of puerarin was determined by HPLC with octadecylsilane-bonded silica gel as the stationary phase, a mixture of methanol and water (25:75) as the mobile phase, 256 nm as the detection wavelength, 1.0 mL/min as the flow rate. Results Contents of total flavonoids in Puerariae Lobatae Radix by ultrasonic method at room temperature, conventional refluxing method and ultrasonic method were15.09%, 14.48%, and 12.71% (n=3), respectively. The contents of puerarin were 4.37%, 4.09%, and 3.80% (n=3), respectively. Contents of total flavonoids in Puerariae Thomsonii Radix were 2.09%, 2.23%, and 2.17% (n=3), respectively. The contents of puerarin were 0.50%, 0.53%, and 0.52% (n=3), respectively. Conclusion Ultrasonic method at room temperature can replace conventional refluxing method for content determinations of total flavonoids and puerarin from Puerariae Lobatae Radix, and ultrasonic method at heating conditions also can replace conventional refluxing method for content determinations of total flavonoids puerarin from Puerariae Thomsonii Radix. Puerarin contents from Puerariae Lobatae Radix and Puerariae Thomsonii Radix in South Anhui Province are all in line with the Pharmacopoeia standards.关键词
供试液制备/葛根/粉葛/总黄酮/葛根素/含量测定Key words
sample preparation/Puerariae Lobatae Radix/Puerariae Thomsonii Radix/total flavonoids/puerarin/content determination分类
医药卫生引用本文复制引用
常跃兴,郭爱灵,陈铎葆,邓云,张荣波,姚斯琪..样品处理方法对皖南葛根和粉葛中总黄酮和葛根素含量的影响[J].中国中医药信息杂志,2017,24(11):78-81,4.基金项目
2015年安徽理工大学引进人才基金项目(11079) （11079）
安徽理工大学大学生创新创业训练计划项目(201610361317) （201610361317）
