中成药2017，Vol.39Issue(10)：1993-1999,7.DOI:10.3969/j.issn.1001-1528.2017.10.001
小檗碱与吴茱萸碱配伍对高胆固醇血症大鼠小肠ACAT2、ApoB48和NPC1L1表达的影响
Effects of berberine-evodiamine compatibility on expressions of intestinal ACAT2, ApoB48 and NPC1L1 in hypercholesterolemic rat
摘要
Abstract
AIM To investigate the effects of berberine-evodiamine compatibility on expressions of intestinal acyl-CoA:cholesterol acyltransferase 2 (ACAT2),apolipoprotein B48 (ApoB48),and Niemann-Pick C1 Like 1 (NPC1L1) proteins in hypercholesterolemic rats.METHODS Fifty SD rats were assigned to control and model groups.After establishing the hypercholesterolemic rat model by feeding high fat and high cholesterol food,forty SD rats were equally divided into model control group,atorvastatin group,berberine-evodiamine compatibility groups (89.2 mg/kg,178.4 mg/kg).After four weeks treatment,serum triglycerides (TG),total cholesterol (TC),low-density lipoprotein cholesterol (LDL-C),high density lipoprotein cholesterol (HDL-C) were detected.Then the contents of cholesterol and β-sitoesterol in serum were determined by GC.The expressions of ACAT2,ApoB48 and NPC1 L1 proteins in the small intestine were assayed with immunohistochemistry technology.RESULTS Serum TC,TG and liver TC were significantly decreased in 89.2 mg/kg and 178.4 mg/kg berberineevodiamine compatibility groups compared with those in the model control group (P ＜ 0.01).Serum LDL-C and liver TC were also significantly decreased in 89.2 mg/kg berberine-evodiamine compatibility group (P ＜ 0.05).GC analysis indicated that the amount of cholesterol level and β-sitoesterol in serum were decreased in 178.4 mg/kg berberine-evodiamine compatibility group (P ＜ 0.05) and 89.2 mg/kg berberine-evodiamine compatibility group (P ＜ 0.01).Immunohistochemistry analysis manifested that the average luminous density of ACAT2,ApoB48 and NPC1L1 proteins in two dosages of berberine-evodiamine compatibility group were descended markedly compared with those in the model control group (P ＜ 0.05 or P ＜ 0.01).CONCLUSION The mechanisms underlying the cholesterol metabolism activity of berberine-evodiamine compatibility are mediated most likely by down-regulating the expressions of intestinal ACAT2,ApoB48 and NPC1 L1 proteins in hypercholesterolemic rats.关键词
小檗碱/吴茱萸碱/高胆固醇血症/大鼠/ACAT2/ApoB48/NPC1L1Key words
berberine/evodiamine/hypercholesterolemia/rat/ACAT2/ApoB48/NPC1L1分类
医药卫生引用本文复制引用
周昕,魏宏,沈涛,魏江平,任佳月,倪恒凡..小檗碱与吴茱萸碱配伍对高胆固醇血症大鼠小肠ACAT2、ApoB48和NPC1L1表达的影响[J].中成药,2017,39(10):1993-1999,7.基金项目
国家自然科学基金(81373545,81703844) （81373545,81703844）
国家重点基础研究发展计划课题(2013CB532005) （2013CB532005）
四川省教育厅课题(17ZB0147) （17ZB0147）
