中成药2017，Vol.39Issue(10)：2045-2049,5.DOI:10.3969/j.issn.1001-1528.2017.10.011
黄芪多糖水提工艺的优化及其体外抗肿瘤活性
Optimization of aqueous extraction for polysaccharides from Astragali Radix and the in vitro antitumor activity
摘要
Abstract
AIM To optimize the aqueous extraction for polysaccharides from Astragali Radix and to evaluate the in vitro antitumor activity.METHODS With extraction temperature,extraction time and solid-liquid ratio as influencing factors,extraction rate of polysaccharides as an evaluation index,the extraction was optimized by uniform design.The effect of polysaccharides on the proliferation of non-small cell lung cancer NCI-H460 cells,the apoptosis rate and cell cycle of NCI-H460 cells,and the expressions of Caspase-3,Bax and Bcl-2 were detected by MTT assay,flow cytometry and Western blot,respectively.RESULTS The optimal conditions were determined to be 100 ℃ for extraction temperature,1 h for extraction time,and 1 ∶ 35 for solid-liquid ratio,the extraction rate of polysaccharides was 3.62％.Compared with the control group,the proliferation of NCI-H460 cells was significandy inhibited in a dose-dependent manner (P ＜ 0.01),the S phase ratio,early apoptosis rate,late apoptosis rate and total apoptosis rate were markedly increased (P ＜ 0.01),and the Caspase-3 expression and Bax/Bcl-2 ratio were also obviously increased (P ＜ 0.01) in the polysaccharides group.CONCLUSION This fast,stable and reliable method can be used for the aqueous extraction for polysaccharides from Astragali Radix,which can significantly inhibit the proliferation of NCI-H460 cells and induce apoptosis of NCI-H460 cells.关键词
黄芪多糖/水提/体外抗肿瘤活性/NCI-H460细胞Key words
polysaccharides from Astragali Radix/aqueous extraction/in vitro antitumor activity/NCI-H460 cells分类
医药卫生引用本文复制引用
阎力君,洪涛,王福玲,李健,田硕,张多,高原,雒江菡..黄芪多糖水提工艺的优化及其体外抗肿瘤活性[J].中成药,2017,39(10):2045-2049,5.基金项目
黑龙江省自然科学基金重点项目(ZD2015007) （ZD2015007）
