中成药2017，Vol.39Issue(10)：2064-2068,5.DOI:10.3969/j.issn.1001-1528.2017.10.015
UPLC-MS/MS法结合模式识别同时测定芪参益气滴丸中11种成分
Simultaneous determination of eleven constituents in Qishen Yiqi Dropping Pills by UPLC-MS/MS combined with pattern recognition
摘要
Abstract
AIM To establish a UPLC-MS/MS combined with pattern recognition method for the simultaneous content determination of danshensu,protocatechuic acid,protocatechuic aldehyde,caffeic acid,rosmarinic,salvianolic acid B,salvianolic acid A,tanshinone Ⅰ,cryptotanshinone,tanshinone ⅡA and ursolic acid in Qishen Yiqi Dropping Pills (Astragali Radix,Salviae miltiorrhizae Radix et Rhizoma,Notoginseng Radix et Rhizoma,etc.).METHODS The analysis of 50％ methanol extract of this drug was performed on a 40 ℃ thermostatic ACQUITY UPLC(R) BEH C1s column (2.1 mm ×50 mm,1.8 μm),with the mobile phase comprising of acetonitrile-0.1％ formic acid flowing at 0.2 mL/min in a gradient elution manner.Then SIMCA14.0 software was adopted in the pattern recognition.RESULTS Eleven constituents showed good linear relationships within their own ranges (r ≥ 0.999 6),whose average recoveries were 98.45％-101.38％ with the RSDs of 1.70％-2.40％.The quality deviations of ten batches of samples were all within 2 std (standard deviation).CON CLUSION This simple,sensitive and accurate method can be used for the quality control of Qishen Yiqi Dropping Pills.关键词
芪参益气滴丸/化学成分/UPLC-MS/MS/模式识别Key words
Qishen Yiqi Dropping Pills/chemical constituents/UPLC-MS/MS/pattern recognition分类
医药卫生引用本文复制引用
周霖,姜晓芳,孙志,左莉华,刘新,贾清泉,张菡,康建,赵杰..UPLC-MS/MS法结合模式识别同时测定芪参益气滴丸中11种成分[J].中成药,2017,39(10):2064-2068,5.基金项目
郑州大学第一附属医院院内青年创新基金项目(2015) （2015）
