中成药2017，Vol.39Issue(10)：2069-2072,4.DOI:10.3969/j.issn.1001-1528.2017.10.016
HPLC法同时测定党参固本丸中7种成分
Simultaneous determination of seven constituents in Dangshen Guben Pills by HPLC
周媛 1陈晶晶 1吴玉华 1虞和永1
作者信息
- 1. 浙江大学医学院附属第四医院药剂科,浙江义乌322000
- 折叠
摘要
Abstract
AIM To establish an HPLC method for the simultaneous content determination of catalpol,acteoside,jionoside B1,martynoside,lobetyolin,methylophiopogonanone A and methylophiopogonanone B in Dangshen Guben Pills (Rehmanniae Radix,Rehmanniae Radix Preparata,Codonopsis Radix,etc.).METHODS The analysis of 50％ methanol extract of this drug was performed on a 30 ℃C thermostatic Agilent TC-C1s column (200 mm ×4.6 mm,5 μm),with the mobile phase comprising of acetonitrile-0.2％ phosphoric acid flowing at 1.0 mL/min in a gradient elution manner,and the detection wavelengths were set at 210,330,269 and 296 nm.RESULTS Seven constituents showed good linear relationships within their own ranges (r ≥0.999 2),whose average recoveries were 96.96％-99.65％ with the RSDs of 0.88％-1.74％.CONCLUSION This simple,accurate and reproducible method can be used for the quality control of Dangshen Guben Pills.关键词
党参固本丸/化学成分/HPLCKey words
Dangshen Guben Pills/chemical constituents/HPLC分类
医药卫生引用本文复制引用
周媛,陈晶晶,吴玉华,虞和永..HPLC法同时测定党参固本丸中7种成分[J].中成药,2017,39(10):2069-2072,4.
