中成药2017，Vol.39Issue(10)：2097-2101,5.DOI:10.3969/j.issn.1001-1528.2017.10.023
MIPs-HPLC法同时测定覆盆子中4种黄酮
Simultaneous determination of four flavonoids in Rubus chingii by MIPs-HPLC
摘要
Abstract
AIM To establish a molecular imprinted polymers (MIPs)-HPLC method for the simultaneous content dertermination of four flavonoids in Rubus chingii Hu.METHODS Fe3O4 magnetic MIPs was added into ethyl acetate fraction solution of R.chingii to prepare mixed MIPs.The analysis of mixed MIPs methanol eluent was performed on a 30 ℃ thermostatic Diamonsil C18 column (250 mm ×4.6 mm,5 μm),with the mobile phase comprising of acetonitrile-0.1％ formic acid flowing at 1.0 mL/min in a gradient elution manner,and the detection wavelength was set at 266 nm.RESULTS Tannins,lilsonide,quercetin and kaempferol showed good linear relationships within the ranges of 2.4-1 232.0 μg (R2 =1)、7.1-3 648.0 μg (R2 =0.999 9)、4.7-4 840.0 μg (R2 =0.999 9)、4.8-2 440.0 μg (R2 =0.999 9),whose average recoveries were 98.94％,99.33％,99.26％ and 98.67％ with the RSDs of 2.04％,1.40％,1.76％ and 1.75％,respectively.CONCLUSION This accurate and reliable method eliminates impurity interference,which can be used for the content determination of flavonoids in R.chingii.关键词
覆盆子/紫云英苷/椴树苷/槲皮素/山柰酚/分子印迹聚合物(MIPs)/HPLCKey words
Rubus chingii Hu/tannins/lilsonide/quercetin/kaempferol/molecular imprinted polymers (MIPs)/HPLC分类
医药卫生引用本文复制引用
马亚娟,白文婷,朱小芳,黄丽萍,谢一辉..MIPs-HPLC法同时测定覆盆子中4种黄酮[J].中成药,2017,39(10):2097-2101,5.基金项目
江西省自然科学基金项目(20142BAB205025) （20142BAB205025）
