浙江医学2017，Vol.39Issue(17)：1408-1412,5.DOI:10.12056/j.issn.1006-2785.2017.39.17.2017-622
桔梗皂苷D联合伊马替尼对K562细胞的增殖抑制作用及机制研究
Effect of platycodin D combined with imatinib on K562 cell in vitro
摘要
Abstract
Objective To investigate the effect and mechanism of Platycodin (PD) combined with imatinib (IM) on chronic myelogenous leukemia K562 cells in vitro.Methods Cultured K562 cells were treated with PD and imatinib alone or in combination.Cell proliferation was examined by CCK8 assay.Cell apoptosis were detected by Annexin V FITC/PI double staining.The change of mitochondrial trans-membrane potential was measured by JC-1 staining.The protein expression of cleaved caspase-3,cleaved caspase-9,PARP,cleaved PARP,Bcr/abl,p-AKT and p-mTOR were detected by Western blot.Results The inhibitory effects of PD combined with imatinib on proliferation and apoptosis of K562 cells were significantly higher than those of the control group or single drug groups (all P＜0.01).The expression of cleaved caspase-3,cleaved caspase-9 and cleaved PARP proteins was significantly up-regulated in the combination group,and the expression of PARP,Bcr/abl,p-AKT and p-mTOR proteins was significantly down-regulated (P＜0.01 or 0.05).Conclusion Platycodin D combined with imatinib can significantly increase the inhibitory effect on cell proliferation and induce apoptosis of K562 cells compared with single drugs,which may be related to Bcr/abl protein and PI3K/AKT/mTOR signaling pathway.关键词
梗皂甙D/伊马替尼/K562/Bcr/abl融合基因/PI3K//AKT/mTOR信号途径Key words
Platycodin D/Imatinib/K562/Bcr/abl fusion gene/PI3K/AKT/mTOR signal pathway引用本文复制引用
代群,葛宇清..桔梗皂苷D联合伊马替尼对K562细胞的增殖抑制作用及机制研究[J].浙江医学,2017,39(17):1408-1412,5.基金项目
国家自然科学基金自助项目(81673755) （81673755）
