浙江医学2017，Vol.39Issue(17)：1417-1420,4.DOI:10.12056/j.issn.1006-2785.2017.39.17.2015-1352
重组IL-4R蛋白对SD大鼠过敏性哮喘干预作用的研究
Intervention of allergic asthma with recombinant IL-4R in rats
摘要
Abstract
Objective To investigate the effect of recombinant IL-4R on allergic asthma in rats.Methods Sixty SD rats in 6 wk of age were randomly divided into model group,IL-4R group,budesonide group (as positive control) and control group with 15 in each.Asthma model was induced with subcutaneous injection of ovalbumin (OVA).Rats in IL-4R group,budesonide group and control group were given intraperitoneal injection of recombinant IL-4R (400μg/d),budesonide (400lμg/d) or normal saline,respectively.The pathological changes of lung tissues were observed with HE staining,the contents of IL-4 and IFN-γ in alveolar lavage fluid were detected by indirect ELISA method.Results Large amount of eosinophil infiltration in the lung tissue was observed in asthma model group,which were significantly attenuated in IL-4R budesonide groups.The contents of IL-4 and IFN-γ in alveolar lavagefluid of IL-4R and budesonide groups were significantly lower than those of asthma model group (P＜0.05).Conclusion The results suggest that recombinant IL-4R can effectively intervene allergic asthma in rats.关键词
IL-4R/IFN-γ/ELISA/过敏性哮喘Key words
IL-4R/IFN-γ/ELISA/Allergic asthma引用本文复制引用
杨光勇,郭海涛,刘茜明,刘莉莉,何光志..重组IL-4R蛋白对SD大鼠过敏性哮喘干预作用的研究[J].浙江医学,2017,39(17):1417-1420,4.基金项目
2011年贵州省社会发展科技攻关项目[黔科合SY字(2011)3020]、贵州省委组织部高层次人才科研条件特助经费(TZJF-2011年28号) （2011）
