作物学报2017，Vol.43Issue(11)：1575-1587,中插1-中插36,49.DOI:10.3724/SP.J.1006.2017.01575
基于寡核苷酸探针套painting的小麦"中国春"非整倍体高清核型及应用
Development and Application of High Resolution Karyotypes of Wheat "Chi-nese Spring" Aneuploids
摘要
Abstract
Oligonucleotide (oligo hereafter) multiplex-based chromosome painting facilitates chromosome identification of both wheat cultivars and its relatives in a simple, easy and high efficient way. In this study, an oligo multiplex containing oligos pAs1-1, pAs1-3, AFA-4, (GAA)10, and pSc119.2-1 developed earlier was used for chromosome painting of 18 accessions from 17 Chinese Spring (CS) aneuploids. The high resolution karyotypes allowed to clearly distinguish individual wheat chromosomes. Fourteen aneuploids had the expected chromosome constitutions whereas the other four had chromosome variations including one with a possible small segmental reciprocal translocation T6AS·6AL-6DL and T6DS·6DL-6AL occurred in N5BT5D. The following analysis on eight landraces, nine cultivars (lines), and one synthetic hexaploid wheat, observed karyotype diversities from 15 chromosomes including six B- (except for 4B), five A- (except for 1A and 3A), and four D-genome (1D, 2D, 4D, and 7D) chro-mosomes. The three widely-used translocations in China, i.e. T1BL·1RS, T6AL·6VS and the reciprocal translocation T1RS·7DL and T7DS·1BL, were clearly detected after only once fluorescence in situ hybridization (FISH) using the oligo multiplex and without genomic in situ hybridization (GISH). This oligo multiplex also produced rich signals in all chromosomes of Triticum monococum,rye cultivar"Jingzhouheimai",durum wheat"Langdon",and Thinopyrum elongatum,and 30 chromosomes of Thi-nopyrum intermedium.The karyotypes of these five species were thus developed.These results indicate that oligo multiplex-based chromosome painting will play active roles on chromosome identifying, and provide a reference for the standard karyotypes of CS aneuploids.关键词
寡核苷酸探针套/染色体painting/染色体多样性/小麦易位系/非整倍体Key words
Oligonucleotide multiplex probe/Chromosome painting/Chromosome diversity/Wheat translocations/Aneuploids引用本文复制引用
王丹蕊,杜培,裴自友,庄丽芳,亓增军..基于寡核苷酸探针套painting的小麦"中国春"非整倍体高清核型及应用[J].作物学报,2017,43(11):1575-1587,中插1-中插36,49.基金项目
本研究由国家自然科学基金项目(31370385)和山西省农业科学院所长青年引导专项(yydzx09)资助.This study was supported by the National Natural Science Foundation of China (31370385) and Institute Director Foundation of Shanxi Academy of Agricultural Sciences for Youth (yydzx09). （31370385）
