作物学报2017，Vol.43Issue(11)：1588-1595,8.DOI:10.3724/SP.J.1006.2017.01588
反义RNA介导GmFAD2-1B基因沉默增强大豆种子中油酸的高效累积
Antisense RNA-mediated GmFAD2-1B Gene Silencing Enhances Accumulation of Oleic Acid in Transgenic Soybean Seeds
摘要
Abstract
The content of oleic acid is usually taken as a crucial indicator of cooking quality and stability for soybean oil. Breed-ing soybean cultivars with high oleic acid has always been considered as one of the principal aims for soybean improvement. In this study, the anti-sense GmFAD2-1B gene from soybean was transformed into the cultivated soybean by Agrobacte-rium-mediated method and several soybean transgenic lines with dramatically increased oleic acid content were obtained.South-ern blot analysis confirmed integration of the anti-sense GmFAD2-1B fragment into the soybean genome with one to five copies of T-DNA insertions in the selected independent transgenic plants. The results of qRT-PCR showed that the foreign anti-sense GmFAD2-1B was mainly expressed in the transgenic seeds and simultaneously resulted in a significant decrease of the endoge-nous GmFAD2-1 mRNA,however,there were no significant differences observed for GmFAD2-1 mRNA contents in the root,leaf, stem and flower tissues of the transgenic plants compared to the non-transgenic ones. Analysis of the fatty acid compositions in-dicated that the content of oleic acid increased to 27.38%-80.42% in twelve transgenic lines. Specifically, in transgenic lines L40 and L72, oleic acid content was raised to 68.91%-80.42% and 65.98%-80.22% respectively, with over 2.65-fold increase com-pared with the wild type Williams 82 (containing 17.8%-22.0% of oleic oil). Concomitantly, linoleic acid and saturated fatty acid were reduced to 4.84%-14.55% and 10.34%-11.16% respectively in the seeds of transgenic lines L40 and L72. However, there was no significant difference in total oil and protein contents, as well as agronomic traits such as maturity period, plant height, leaf shape, flower color, pod height and weight per 100 seeds between the transgenic lines and non-transformed controls.关键词
大豆/油酸/GmFAD2-1B/农杆菌介导转化法Key words
Soybean/Oleic acid/GmFAD2-1B/Agrobacterium-mediated transformation引用本文复制引用
杨静,邢国杰,牛陆,贺红利,杜茜,郭东全,袁英,杨向东..反义RNA介导GmFAD2-1B基因沉默增强大豆种子中油酸的高效累积[J].作物学报,2017,43(11):1588-1595,8.基金项目
本研究由国家转基因生物新品种培育科技重大专项(2016ZX08004-003)和国家自然科学基金项目(31671764)资助.This study was supported by the National Major Project for Developing New GM Crops (2016ZX08004-003) and the National Natural Sci-ence foundation of China (31671764). （2016ZX08004-003）
