作物学报2017，Vol.43Issue(11)：1611-1621,11.DOI:10.3724/SP.J.1006.2017.01611
利用水稻MAGIC群体关联定位抽穗期和株高QTL
Mapping of QTLs for Heading Date and Plant Height Using MAGIC Popula-tions of Rice
摘要
Abstract
Association analysis was performed to map QTLs for heading date (HD) and plant height (PH) using three multi-parent advanced generation inter-cross (MAGIC) populations including two four-parent- and one eight-parent-derived populations (DC1, DC2, and 8way), and their composite populations (DC12=DC1+DC2, RMPRIL=DC12+8way) derived from eight elite indica lines. Using the phenotypic data of HD collected in the two sites (Jiangxi and Shenzhen) across two years (2015 and 2016), PH collected in the two sites(Jiangxi and Shenzhen)in 2016,and SNP genotypic data of rice 55K SNP chip,three HD QTLs,qHD3, qHD6 and qHD8 were identified in the same regions of DTH3, Hd3a, and Ghd8, respectively. And five PH QTLs (qPH1.1, qPH1.2,qPH1.3,qPH4,and qPH6)were also identified,of which qPH1.1 and qPH1.2 were located in the adjacent regions of Psd1 and sd1.The other three PH QTLs were new loci which were detected only in one population under one environment show-ing larger effects of genetic background and environment. Different MAGIC populations had different mapping effects for HD and PH. Using eight-parent-derived populations such as 8way, DC12 and RMPRIL not only detected more HD and PH QTLs (5, 5, and 6) but also had higher mapping resolution than four parents-derived populations such as DC1 and DC2 (2 and 4), showing an obvious advantage in two aspects of QTL mapping: highly significant level and closed to known genes. Especially, joint analyses of different populations such as DC12 and RMPRIL had significant advantage in QTL mapping.关键词
水稻/MAGIC群体/抽穗期/株高/关联分析/QTLKey words
Rice/MAGIC population/Heading date/Plant height/Association analysis/Quantitative trait locus/loci (QTL)引用本文复制引用
申聪聪,朱亚军,陈凯,陈慧珍,吴志超,孟丽君,徐建龙..利用水稻MAGIC群体关联定位抽穗期和株高QTL[J].作物学报,2017,43(11):1611-1621,11.基金项目
本研究由国家高技术研究发展计划(863计划)项目(2014AA10A601), 农业部引进国际先进农业科学技术计划(948计划)项目(2016-X16),深圳孔雀团队计划(20130415095710361)和中国农业科学院科技创新工程团队项目资助.This study was supported by the National High Technology Research and Development Program of China (863 Program) (2014AA10A601), The Program of Introducing International Super Agricultural Science and Technology (948 Program) (2016-X16), Shenzhen Peacock Plan (20130415095710361), and the Scientific and Technological Innovation Project of Chinese Academy of Agricultural Sciences. （863计划）
