作物学报2017，Vol.43Issue(11)：1622-1631,10.DOI:10.3724/SP.J.1006.2017.01622
利用分子标记辅助选择聚合水稻Pi-ta、Pi-b和Wx-mq基因
Pyramiding Pi-ta, Pi-b, and Wx-mq Genes by Marker-assisted Selection in Rice (Oryza sativa L.)
摘要
Abstract
In recent years, japonica rice varieties Nanjing 46, Nanjing 5055, and Nanjing 9108 were planted in a large area in Jiangsu province because of their good eating quality. However, these varieties are not resistant to rice blast, and there is no japonica rice variety with good eating quality and suitable for planting in Huaibei area,Jiangsu province.The goal of modern rice breeding is to develop new varieties with good quality, high yield and multi-resistance. Marker-assisted selection combined with traditional breeding methods can greatly improve breeding efficiency. In this study, the high yield rice variety "Wujing 15" con-tained two blast resistance genes of Pi-ta and Pi-b was used as gene recipient to be crossed with the rice variety"Nanjing 5055", which carries the low-amylose content gene Wx-mq.We developed two multiple PCR systems to identify resistance and suscepti-ble alleles on Pi-ta and Pi-b loci and the Tetra-primer Amplification Refractory Mutation PCR System for Wx-mq gene. MAS technique was adopted in combination with field phenotyping of agronomic traits, blast resistance identification, and classification of the grain endosperm appearance.Finally,a new japonica line,tentatively designated"Nanjing 0051",was bred,which showed excellent agronomic traits and adaptation in the Huaibei area. The results indicate that the three PCR systems developed in this study are applicable and effective in rice breeding to improve blast resistance and grain quality. The success of breeding "Nanjing 0551" is an example for using MAS technique to pyramid favorable genes and facilitate breeding process in rice.关键词
水稻/稻瘟病/低直链淀粉含量/Pi-ta/Pi-b/Wx-mq/分子标记辅助选择Key words
Rice/Blast/Low-amylose content/Pi-ta/Pi-b/Wx-mq/Marker-assisted selection引用本文复制引用
姚姝,陈涛,张亚东,朱镇,赵庆勇,周丽慧,赵凌,赵春芳,王才林..利用分子标记辅助选择聚合水稻Pi-ta、Pi-b和Wx-mq基因[J].作物学报,2017,43(11):1622-1631,10.基金项目
本研究由江苏省农业科技自主创新基金项目(CX[12]1003), 江苏省重点研发计划项目(BE2016370)和国家现代农业产业技术体系建设专项(CARS-01-62)资助.This study was supported by the Agricultural Science and Technology Independent Innovation Fund of Jiangsu Province (CX [12]1003), the Key Research and Development Projects of Jiangsu Province (BE2016370), and China Agriculture Research System (CARS-01-62). （CX[12]1003）
