作物学报2017，Vol.43Issue(11)：1632-1642,11.DOI:10.3724/SP.J.1006.2017.01632
假禾谷镰孢侵染小麦后3种植物激素相关基因的差异表达分析
Differential Expression of Three Plant Hormone Related Genes in Wheat In-fected by Fusarium pseudograminearum
摘要
Abstract
Fusarium pseudograminearum is a pathogenic fungus in wheat newly found in China.The purpose of this study was to disclose the responses of genes involved in the hormone signals pathway after F.pseudograminearum infection.Wheat cultivar Zhoumai 24 was infected by the wild-type strain WZ2-8A, and the seedling samples at five and fifteen days after inoculation (DAI) were subject to transcriptome analysis by RNA-seq method. The selected differentially expressed genes were subsequently vali-dated by qRT-PCR assay.The infection of F.pseudograminearum had a negative impact on wheat growth with significantly de-creased root length, seedling height, and fresh weights of root and shoot. A total of 29 genes expressed differentially in the signal transduction pathways of auxin, cytokinin (CTK) and abscisic acid (ABA). At 5 DAI, 11 genes were expressed differentially compared with the control group (without WZ2-8A inoculation), in which two and nine genes were up- and down-regulated, re-spectively. At 15 DAI, 25 genes were expressed differentially, including eight up-regulated and seventeen down-regulated genes. In the auxin signaling pathway, auxin input transporter AUX1 was differentially expressed, influencing polar transport of auxin and disturbing cell elongation of wheat roots. In the CTK signaling pathway, the up-regulating role of B-ARR suggests that it could facilitate CTK signal transduction to inhibit cellular division, resulting in weaker wheat growth along with the interaction of the auxin signaling pathway. In the ABA signaling pathway, ABA receptor PYR/PYL was down-regulated, while the negative regulator PP2C was up-regulated. ABA plays a negative role in regulating host resistance to fungi and bacteria and its transduction has the antagonistic action against the jasmine acid (JA) / ethylene (ET) pathway, i.e., the repression of ABA signaling pathway might result in the opening of JA/ET pathway. These results of transcriptome analysis were generally validated by the qRT-PCR evidence,indicating that inhibition of ABA signal transduction after F.pseudograminearum infection might be the physiological basis of moderate resistance to the fungus in Zhoumai 24.关键词
小麦/假禾谷镰孢/生长素/细胞分裂素/脱落酸/基因差异表达Key words
Wheat/Fusarium pseudograminearum/Auxin cytokinin/Abscisic acid/Gene differential expression引用本文复制引用
李永辉,陈琳琳,孙炳剑,王利民,邢小萍,袁虹霞,丁胜利,李洪连..假禾谷镰孢侵染小麦后3种植物激素相关基因的差异表达分析[J].作物学报,2017,43(11):1632-1642,11.基金项目
本研究国家公益性行业(农业)科技专项经费(201503112)资助.This study was supported by China Special Fund for Agro-scientific Research in the Public Interest (201503112). （农业）
