作物学报2017，Vol.43Issue(11)：1643-1649,7.DOI:10.3724/SP.J.1006.2017.01643
利用BSR-Seq定位小麦品种郑麦103抗条锈病基因YrZM103
Mapping Stripe Rust Resistance Gene YrZM103 in Wheat Cultivar Zhengmai 103 by BSR-Seq
摘要
Abstract
Zhengmai 103 is a new wheat cultivar highly resistant to stripe rust. In order to detect the stripe rust resistance gene in Zhengmai 103, a segregating population was developed by making cross between Zhengmai 103 and a highly stripe rust suscepti-ble wheat cultivar Nongda 399. The 214 F2derived F2:3 progenies were inoculated with mixed prevailing Pst races CYR32, CYR33 and CYR34 (V26) in field condition for disease resistance evaluation at adult plant-stage. Genetic analysis revealed that the stripe rust resistance of Zhengmai 103 is controlled by a single gene,temporarily designated YrZM103.By applying RNA-seq with bulked segregant analysis(BSA),six polymorphic markers were developed to map YrZM103 on chromosome 7BL flanked by markers ZM215 and ZM221 with genetic distances of 6.9 cM and 11.8 cM,respectively.Comparative genetic mapping indi-cated YrZM103 was located on different genetic interval from that of known stripe rust resistance genes on 7BL.关键词
郑麦103/条锈病/分子标记/BSR-Seq/小麦Key words
Zhengmai 103/Stripe rust/Molecular marker/BSR-Seq/Wheat引用本文复制引用
张怀志,贾联合,曹廷杰,刘志勇,谢菁忠,陈永兴,刘旭,王勇,闫素红,杨兆生,赵虹,王西成..利用BSR-Seq定位小麦品种郑麦103抗条锈病基因YrZM103[J].作物学报,2017,43(11):1643-1649,7.基金项目
本研究由国家重点研发计划项目(2017YFD0101802)资助.This study was supported by the National Key Research and Development Program of China (2017YFD0101802). （2017YFD0101802）
