作物学报2017，Vol.43Issue(11)：1658-1666,9.DOI:10.3724/SP.J.1006.2017.01658
涝渍对Bt棉棉蕾杀虫蛋白表达含量及氮代谢影响
Effects of Waterlogging on Bt Protein Content and Nitrogen Metabolism in Square of Bt Cotton
摘要
Abstract
Using Bt cotton conventional cultivar Sikang 1 and hybrid Sikang 3 as test materials, the experiments of water stress at different levels (relative soil water capacity at 90%, 100%, and 2 cm waterlogging above the soil surface respectively) and 90% soil water capacity remained 24-120 hours were conducted to investigate the square Bt insecticidal protein expression and nitro-gen metabolism in bud developmental stage. The soil waterlogging treatments with three levels reduced the square Bt insecticidal protein content by 36.5%, 42.1%, and 51.4% for conventional cultivar Sikang 1 and by 19.2%, 57.2%, and 64.5% for hybrid Si-kang 3, respectively. The continuous stress of 96 h in the 90% soil water capacity treatment decreased the square Bt insecticidal protein content significantly, which was 20.8% for Sikang 1 and 17.6% for Sikang 3, the longer the stress sustained, the more the Bt toxin content declined. The soluble protein content, NR, GOT, GPT, GS, and GOGAT activities decreased, and the free amino acids content, peptidase and protease activities increased under different levels of soil waterlogging and continuous stress of 90% soil water capacity stress. Therefore, the reduced protein synthesis ability, and enhanced protein degradation ability may decrease the square soluble protein content including Bt insecticidal protein content, resulting in reduction of the square insect resistance.关键词
Bt棉/涝渍/杀虫蛋白/氮代谢Key words
Bt cotton/Water logging/Insecticidal protein/Nitrogen metabolism引用本文复制引用
李远,李亚兵,胡大鹏,王俊,衡丽,张祥,陈源,陈德华..涝渍对Bt棉棉蕾杀虫蛋白表达含量及氮代谢影响[J].作物学报,2017,43(11):1658-1666,9.基金项目
本研究由国家自然科学基金项目(31471435, 31301263, 31671613), 江苏省三新工程项目[SXGC(2014)317, (2016)320], 江苏省高等教育优势学科发展计划(PAPD)和中国农业科学院棉花研究所植棉技术标准化研究与示范团队项目(2016PCTS-1)资助.This study was supported by the National Natural Science Foundation of China (31471435, 31301263, 31671613), Jiangsu "Three-innovation" Agricultural Project [SXGC(2014)317, (2016)320], the Priority Academic Program Development of Jiangsu Higher Education Institutions (PAPD), and the Project of Planting Cotton Technology Standardization Research and Demonstration. （31471435, 31301263, 31671613）
