植物学报2017，Vol.52Issue(5)：615-621,7.DOI:10.11983/CBB16219
不同诱导子对怀牛膝细胞生长及多糖含量的影响
Effect of Different Elicitors on Cell Growth and Polysaccharides Content of Achyranthes bidentata
摘要
Abstract
To enhance the polysaccharides content of Achyranthes bidentata suspension cell,yeast extract,Pleurotus citrinopileatus and salicylic acid were added at different concentrations or at different times as the elicitor.With 2.5％ (v/v) yeast elidtor added at 12 days,the cell dry weight was the greatest,reaching 46.75 g·L-1;polysaccharides content was less,5.76 mg·g-1.With 5 mgGE·L-1 fungal elicitor added after culture for 9 days,polysaccharides content could reach 6.56 mg·g-1;with cell dry weight 28.3 g·L-1.With 1 mg·g-1SA elicitation,the effect was a little inferior to the above two effects.关键词
怀牛膝/细胞悬浮培养/诱导子/多糖Key words
Achyranthes bidentata/cell suspension culture/elicitor/polysaccharides引用本文复制引用
李萍,许言,张晓丽,李炯,赵喜亭,李明军..不同诱导子对怀牛膝细胞生长及多糖含量的影响[J].植物学报,2017,52(5):615-621,7.基金项目
国家中医药行业科研专项(No.201407005-08)、河南省创新型科技人才队伍建设工程(No.C20130037)和新乡市科技创新平台建设项目(No.CP1401) （No.201407005-08）
