组织工程与重建外科杂志2017，Vol.13Issue(5)：287-290,298,5.DOI:10.3969/j.issn.1673-0364.2017.05.013
趋化因子SDF-1促进基于内源性干细胞归巢的初步研究
A Preliminary Study of SDF-1 in Stem Cell Homing-guided Tissue Regeneration
黄晓 1陈晖 2刘玉健 2汪俊2
作者信息
- 1. 200001 上海市 上海市口腔病防治院儿童口腔科
- 2. 200011 上海市 上海交通大学医学院附属第九人民医院儿童口腔科,上海市口腔医学重点实验室
- 折叠
摘要
Abstract
Objective To investigate the chemotaxis of stromal cell derived factor-1 (SDF1) in vitro and in vivo. Methods Bone marrow mesenchymal stem cells (BMSCs) were cultivated from SD rats. Transwell test was used to evaluate the influences of different concentration of SDF1 on chemotaxis of BMSC. MTT test was used to analyze the effects of different concentration of SDF1 on the cell viability of BMSC. 100 ng/mL SDF1-loaded PLGA was implanted in nude mice to study the the chemotaxis of SDF1 in vivo. Results All 4 groups of SDF1 (10 ng/mL, 50 ng/mL, 100 ng/mL and 200 ng/mL) recruited more BMSC than the control group, and 100 ng/mL SDF1 showed better results than the other groups. 200 ng/mL SDF1 deceased cell viability of BMSC on day 1, 4 and 7, while 10 ng/mL, 50 ng/mL and 100 ng/mL SDF1 had no effects on the the viability of BMSC. In vivo analysis showed that 100 ng/mL SDF1 could promote cell recruitment. Conclusion 100 ng/mL SDF1 could effectively promote BMSC recruitment without influence on cell viability in vitro and 100 ng/mL SDF1 could promote cell homing in vivo.关键词
基质细胞衍生因子1/骨髓间充质干细胞/干细胞募集/趋化作用Key words
Stromal cell derived factor-1/Bone marrow mesenchymal stem cells/Stem cell recruitment/Chemotaxis分类
生物科学引用本文复制引用
黄晓,陈晖,刘玉健,汪俊..趋化因子SDF-1促进基于内源性干细胞归巢的初步研究[J].组织工程与重建外科杂志,2017,13(5):287-290,298,5.
