重庆医学2017，Vol.46Issue(29)：4036-4039,4.DOI:10.3969/j.issn.1671-8348.2017.29.002
孕期和哺乳期暴露壬基酚对仔鼠肝脏细胞色素P450 2E1 mRNA及蛋白表达的影响
Effects of nonyphenol exposure during pregnant and lactation period on expressions of CYP2E1 mRNA and protein of hepatic tissues in offspring rats
摘要
Abstract
Objective To investigate the effects of nonyphenol (NP)exposure during pregnant and lactation period on expression of cytochrome P450 2E1 (CYP2E1) mRNA and protein of hepatic tissues in offspring rats.Methods Pregnant rats were assigned to four groups:the exposure groups received gavage with NP at dose levels of 50 mg · kg-1 · d-1 (low dose group),100 mg · kg-1 · d-1 (medium dose group),200 mg · kg-1 · d-1 (high dose group) and the control group was treated with corn oil alone,NP exposure time was limited from gestational day 6 to postnatal day 21.The newborn rats were sacrificed at 90 days after birth,followed by blood collection and serum separation.Then,serum biochemical indicators of liver function and lipid levels were detected.Glutathione peroxidase (GSH-PX),superoxide dismutase (SOD) activities,malondialdehyde (MDA) level and CYP2E1 mRNA and protein levels were determined in hepatic tissues.Pathologic changes in hepatic tissues were observed with HE staining.Results Compared with the control group,the aspartate aminotransferase (ALT),alanine aminotransferase (AST) levels and AST/ALT ratio of offspring rats in exposure groups were increased in a dose-dependent manner (P＜0.05),as well as serum TG,TC and LDL-C levels were increased (P＜0.05).The liver tissue structure of the control group was normal.The hepatic sinus of the medium dose group was showed mild expansion and inflammatory cellular infiltration.Otherwise,the liver of high dose group had a large amount of lipid droplets.Compared with the control group,SOD and GSH-PX activities were obviously decreased,while MDA level was significantly decreased in each exposure group (P＜0.05).CYP2E1 mRNA and protein expression levels of medium and high dose groups were higher than those in the control group (P＜0.05).Conclusion Exposure to NP during gestation and lactation period can induce lipid metabolism disorders and inflammatory lesions in hepatic tissues of offspring rats,and it maybe associated With up-regulation of CYP2E1 expression.关键词
壬基酚/孕期/哺乳期/肝脏/细胞色素P450Key words
nonylphenol/gestation period/suckling period/liver/cytochrome P450分类
医药卫生引用本文复制引用
罗娅,俞捷,杨雪峰,杨雪松,杨静,许洁..孕期和哺乳期暴露壬基酚对仔鼠肝脏细胞色素P450 2E1 mRNA及蛋白表达的影响[J].重庆医学,2017,46(29):4036-4039,4.基金项目
国家自然科学基金资助项目(81360439,81560527) （81360439,81560527）
贵州省科技厅基金资助项目(黔科合LH字[2014]7543,[2015]7521号,黔科合J字[2014]2177号,[2014]2185号) （黔科合LH字[2014]7543,[2015]7521号,黔科合J字[2014]2177号,[2014]2185号）
贵州省优秀青年科技人才([2017]5612) （[2017]5612）
贵州省卫生计生委科学技术基金(gzwjkj2016-1-045,gzwjkj2017-1-053) （gzwjkj2016-1-045,gzwjkj2017-1-053）
遵义医学院硕士科研启动基金资助项目(F-835) （F-835）
遵义医学院重点学科建设经费基金资助项目(2015). （2015）
