结直肠肛门外科2017，Vol.23Issue(4)：425-431,7.
不同压力下CO2气腹处理对结肠癌HT-29细胞增殖及侵袭能力的影响
The effect of CO2 pneumoperitoneum on the proliferation and invasion of HT-29 colon cancer cells under different pressures
摘要
Abstract
Objective To explore the effect of CO2 pneumoperitoneum on the proliferation and invasion of HT-29 colon cancer cells under different pressures.Methods With an in vitro artificial pneumoperitoneum model,HT-29 cells were exposed to CO2-insufflation of three pressure groups(5,10 and 15 mmHg) for 1h.The HT-29 cells in the control group were conventionally cultured.The pH value of cell culture medium was measured immediately after the intervention.After 24 h of routine culture,HT-29 cells were inoculated in nude mice to establish in vivo model (6 mice in each group).In vitro,the cell adhesion rate and immunocytochemistry assay of Ecadherin expression were detected by using Methylthiazolyl tetrazolim(MTT).After 5 days of conventional culture,the growth curves of the cells in each group were drown.After 3 weeks,the metastasis of peritoneal tumor in nude mice was examined by laparotomy and the average mass of tumor was measured.The expression of E-cadherin in tumor sections was detected by immunohistochemistry.Results After 1 hour of the intervention,the pH value of the cell culture fluid in the three observation groups was significantly lower than the control group (P ＜ 0.05).Compared with the control group,the cell adhesion rate,the growth status of tumor in peritoneal cavity,and the expression level of E-cadherin in the 10 mmHg group and the 15 mmHg group were significantly lower (P ＜ 0.05),whilethey were not significantly different between the 5 mmHg group and the control group (P ＞ 0.05).With the increasing of the p ressure of the pneumoperitoneum,there was a decending trend.Conclusion The treatment of 10 mmHg and 15 mmHg CO2 pneumoperitoneum in vitro can decrease the proliferation ability of HT-29 colon cancer cells,and without influencing the invasion ability.While 5 mmHg CO2 pneumoperitoneum has no significant effect on HT-29 cells.关键词
CO2气腹/结肠癌细胞/增殖/侵袭Key words
CO2 pneumoperitoneum/colon cancer cells/proliferation/invasion分类
医药卫生引用本文复制引用
王献哲,苏棋,何萍,梁思远,李辉,徐艳松,唐卫中..不同压力下CO2气腹处理对结肠癌HT-29细胞增殖及侵袭能力的影响[J].结直肠肛门外科,2017,23(4):425-431,7.基金项目
广西壮族自治区教育厅资助项目(201202ZD019) （201202ZD019）
广西科技基础条件平台建设项目(15-235-06) （15-235-06）
