湖南中医药大学学报2017，Vol.37Issue(10)：1052-1055,4.DOI:10.3969/j.issn.1674-070X.2017.010.002
蜘蛛丝蛋白促凝血作用及其机制的初步探讨
Preliminary Discussion on Procoagulant Role and Mechanism of Spider Silk Protein
摘要
Abstract
Objective To investigate the procoagulant role of spider silk protein and discuss its mechanism. Methods To compare the clotting time in vitro and hemostatic time of spider silk protein group, Yunnan Baiyao group and saline group through coagulation experiment conducted in vitro (coagulation method, tube method and recalfication time) and hemostatic ex-periment in wound surfaces (skin, liver, and femoral artery) in SD rats. Activated partial thromboplastin in time (APTT), pro-thrombin time (PT) and thrombin time (TT) were evaluated by solidification method in rats of each group. Results The clotting time in vitro and hemostatic time of spider silk protein group and Yunnan Baiyao group were obviously shortened compared with that of saline group (P<0.01 or P<0.05). Moreover, APTT, PT and TT were also obviously shortened compared with those of saline group (P<0.01 or P<0.05). Conclusion Spider silk protein shows obvious blood clotting effect. Activating endogenous coagulation and extrinsic coagulation may be one of its procoagulant mechanisms.关键词
蜘蛛丝蛋白/促凝血试验/凝血时间/止血时间Key words
spider silk protein/procoagulant test/clotting time/hemostatic time分类
医药卫生引用本文复制引用
范钊坤,秦甜,郑洁,曹泽标,周小青..蜘蛛丝蛋白促凝血作用及其机制的初步探讨[J].湖南中医药大学学报,2017,37(10):1052-1055,4.基金项目
国家自然科学基金(81373702,81473567,81202632),教育部博士点基金(20124323120003),湖南省自然科学基金(13JJ3097),湖南省教育厅科研项目(14B134,15K092),湖南中医药大学研究生创新项目(2014cx04). （81373702,81473567,81202632）
