湖南中医药大学学报2017，Vol.37Issue(10)：1063-1069,7.DOI:10.3969/j.issn.1674-070X.2017.010.004
健脾补土组方对体外神经元低氧/复氧后PI3K、Akt、Caspase-3表达的影响
Effect of Jianpi Butu Formula on the Expression of PI3K, Akt, Caspase 3 and Apoptosis in Neurons in Vitro Induced by Hypoxia/Reoxygenation
摘要
Abstract
Objective To observe the effect of Jianpi Butu (tonifying spleen) formula on apoptosis and expression of PI3K, Akt and Caspase3 in neonatal SD neurons cultured in vitro induced by hypoxia/reoxygenation, to explore the mechanism of reducing neuronal apoptosis. Methods Primary cultured neurons of newborn SD rats were randomly divided into normal serum control group, hypoxia model group, NGF group, Jianpi Butu formula group. Specimens to modeling were induced by hypoxia for 24 h, reoxygenation for 4 h. The neuronal apoptosis was induced by flow cytometry. The expression of PI3K,Akt and Caspase3 mRNA was determined by qPCR. The expression of PI3K, Akt and Caspase3 protein was detected by im-munocytochemistry and Western blot. Results Compared with normal control group, the rate of neuronal apoptosis in hypoxia model group increased significantly, the expression of Akt mRNA and protein reduced obviously, and the expres-sion of Caspase3 protein and mRNA increased significantly. Compared with the hypoxia model group, the rate of neuronal apoptosis in NGF group, Jianpi Butu formula group decreased significantly, and the expression of Akt mRNA and protein in-creased obviously, and the expression of Caspase3 mRNA and protein decreased significantly (P<0.01). Compared with the nerve growth factor group, the neuronal apoptosis rate in Jianpi Butu group decreased significantly, the expression of Akt mRNA and protein increased obviously, and the expression of Caspase3 mRNA and protein decreased significantly. Con-clusion Jianpi Butu formula could reduce the apoptosis of neuron after cerebral ischemia. Its mechanism may be related to the up-regulated expression of PI3K and Akt, the ultimate expression of inhibitory factor Caspase3 plays a key role in cell apoptosis.关键词
健脾补土/神经元/凋亡/磷脂酰肌醇-3激酶/蛋白激酶-B/半胱天冬酶-3Key words
tonifying spleen/neurons/apoptosis/PI3K/FAK/Caspase3分类
医药卫生引用本文复制引用
陈昱文,李花,刘旺华,周小青,郑彩杏,金梦,范元硕,李诚均,颜艳艳..健脾补土组方对体外神经元低氧/复氧后PI3K、Akt、Caspase-3表达的影响[J].湖南中医药大学学报,2017,37(10):1063-1069,7.基金项目
国家自然科学基金(81202632、81373702、81473567),教育部博士点基金(20124323120003),湖南省自然科学基金(13JJ3097),湖南省教育厅科研项目(14B134),湖南省高校创新平台开放基金(15K092),湖南中医药大学中诊开放基金(2015ZYZD30),湖南省研究生科研创新项目(CX2016B368). （81202632、81373702、81473567）
