检验医学与临床2017，Vol.14Issue(18)：2667-2669,2672,4.DOI:10.3969/j.issn.1672-9455.2017.18.005
IL-3-PE38KDEL转染CIK细胞对HL60细胞体内外杀伤作用的实验研究
The cytotoxicity of CIK cells transfected with IL-3-PE38KDEL fusion gene against HL60 cells
摘要
Abstract
Objective To investigate the anti-tumor effect of cytokine induced-killer (CIK) cells transfected with IL-3-PE38KDEL fusion gene on HL60 cells in vitro and in vivo.Methods IL-3-PE38KDEL fusion gene was transfected into CIK cells by liposome mediate.The phenotype of CIK cells was detected by flow cytometry.The concentration of IFN-γ and TNF-α in supernatant of CIK cells was determined by enzyme-linked immunosorbent assay (ELISA).Cytotoxicity of CIK cells transfected with IL3-PE38KDEL gene against HL60 cells was detected by MTS assay.The human leukemia HL60 cell line subcutaneous xenograft tumor model in nude mice was established.CIK cells transfected with IL-3-PE38KDEL,CIK cells transfected with empty vector,non-transfeced CIK cells and normal saline were given respectively to study the growth inhibitory effect on the transplanted tumor in nude mice.Results The phenotype and the level of cytokine remained unaltered in the IL-3-PE38KDEL gene transfected CIK cells.By comparison with non-transfected CIK cells and those transfected by empty vector,CIK cells transfected with IL-3-PE38KDEL had a significantly high anti-tumor cytotoxic activity against HL60 cells in vitro,and decreased tumor weight.Conclusion IL-3-PE38KDEL fusion gene transfection can enhance the anti-tumor activity of CIK cells.关键词
IL-3-PE38KDEL/CIK细胞/HL60细胞/裸鼠Key words
IL-3-PE38KDEL/cytokine induced-killer cells/HL60/nude mice引用本文复制引用
沈燕,程诗迪,张萍,白凤霞,娄世锋..IL-3-PE38KDEL转染CIK细胞对HL60细胞体内外杀伤作用的实验研究[J].检验医学与临床,2017,14(18):2667-2669,2672,4.基金项目
重庆市自然科学基金项目(CSCT,2009BB5266) （CSCT,2009BB5266）
重庆市科技攻关重点项目(CSTC,2009AB5215). （CSTC,2009AB5215）
