临床神经病学杂志2017，Vol.30Issue(5)：369-372,4.
帕金森病模型小鼠脾悬液中辅助性T细胞17和髓系抑制性细胞数量的变化及其意义
Changes and their significances of the numbers of T helper 17 cells and Myeloid-derived suppressor cells in the spleen suspension of model mouse with Parkinson's disease
摘要
Abstract
Objective To observe the changes and their significances of the numbers of T helper 17 cells (Th17) and Myeloid-derived suppressor cells (MDSC) in the spleen suspension of model mouse with Parkinson 's disease ( PD).Methods Forty C57BL/6 mouse were randomly divided into PD group ( A group ), PD +Gemcitabine group (B group), Gemcitabine group (C group) and control group (D group), with 10 mouse in each group.Mouse in group A and B were injected intraperitoneally with 1-Methyl-4-phenyl-1, 2, 3, 6-tetrahydropyridine (MPTP) (30 mg/kg) for 7 d continuously.Mouse in group B and C were injected intraperitoneally with Gemcitabine (120 mg/kg) at 1 d and 7 d.After behavioral experiment at 8 d, the number of tyrosine hydroxylase (TH) positive cells in the substantia nigra and the cell percentage of Th 17 and MDSC in the spleen suspension of mouse in each group were detected .Results Compared with before modeling , the turning around time and pole climbing time of mouse in A group and B group after modeling were significantly longer , while the suspension time was significantly shortened (all P<0.05).Compared with C group and D group , the turning around time and pole climbing time of mouse in A group and B group after modeling were significantly longer , while the suspension time was significantly shortened (all P<0.05).Compared with C group and D group, the numbers of TH positive cells in substantia nigra of mouse in A group and B group were significantly reduced ( all P<0.05 ) .Compared with C group and D group , the cell percentages of Th 17 and MDSC in the spleen suspension of mouse in A group and B group were significantly increased (all P<0.05).The cell percentage of Th17 in the spleen suspension of mouse in B group was significantly higher than A group (P<0.05), while the cell percentage of MDSC in the spleen suspension of mouse in A group was significantly higher than B group ( P<0 .05 ) .Pearson correlation analysis results showed that there was no correlation between the cell percentages of Th 17 and MDSC in A group and B group ( r=-0.319, r=0.04, all P>0.05).Conclusions Th17 and MDSC are increased in PD model mice .They may be involved in the occurrence and development of PD .MDSC maybe plays an immune role in inhibition of Th 17 amplification .关键词
帕金森病/辅助性T细胞17/髓系抑制性细胞/1-甲基-4-苯基-1,2,3,6-四氢吡啶/吉西他滨Key words
Parkinson's disease/T helper 17 cells/Myeloid-derived suppressor cells/1-Methyl-4-phenyl-1,2,3,6-tetrahydropyridine/Gemcitabine分类
医药卫生引用本文复制引用
陈思远,舒扬,赵峰,于明..帕金森病模型小鼠脾悬液中辅助性T细胞17和髓系抑制性细胞数量的变化及其意义[J].临床神经病学杂志,2017,30(5):369-372,4.基金项目
江苏省自然科学基金(BK20160548) （BK20160548）
江苏省"333 工程"科研项目(BRA2014173) （BRA2014173）
江苏省"六大人才高峰"科研项目(WSN038) （WSN038）
镇江市重点研发计划(SH2016032) （SH2016032）
镇江市社会发展科技计划项目(SH2015047) （SH2015047）
